Overview
- Product nameAnti-Involucrin antibody [SY5], predilutedSee all Involucrin primary antibodies ...
- DescriptionMouse monoclonal [SY5] to Involucrin, prediluted
- Tested applicationsIHC-P more details
- Species reactivityReacts with: Rat, Dog, Human, Pig, Monkey, Owl, Gorilla
- Immunogen
Human involucrin from keratinocytes
- EpitopeCodon 421-568
- Positive controlHuman skin. LS174T cells.
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.5M Tris HCl, stabilizing buffer, pH 7.6 -
Concentration information loading... - PurityProtein G purified
- Purification notesab74181 was purified from ascites fluid.
- Clonality Monoclonal
- Clone numberSY5
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab74181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P |
Note: Enzyme digestion is better than citrate buffer based epitope unmasking.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionPart of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
- Tissue specificityKeratinocytes of epidermis and other stratified squamous epithelia.
- Sequence similaritiesBelongs to the involucrin family.
- Post-translational
modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. - Cellular localizationCytoplasm. Constituent of the scaffolding of the cornified envelope.
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Database links
- Entrez Gene: 609423 Dog
- Entrez Gene: 3713 Human
- Entrez Gene: 407242 Pig
- Entrez Gene: 407242 Pig
- Entrez Gene: 60583 Rat
- Omim: 147360 Human
- SwissProt: P18174 Dog
- SwissProt: P07476 Human
- SwissProt: P18175 Pig
- SwissProt: P48998 Rat
- Unigene: 516439 Human
- Unigene: 142591 Rat
see all
Target information above from: UniProt accession
P07476
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- INVO_HUMAN antibodyinvolucrin antibodyivl antibody
Anti-Involucrin antibody [SY5], prediluted images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Involucrin antibody [SY5], prediluted (ab74181)](http://a.abcam.com/ps/datasheet/Images/74/ab74181/IHC involucrin.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Involucrin antibody [SY5], prediluted (ab74181)Formalin-fixed, paraffin-embedded human skin stained with ab74181 using peroxidase-conjugate and DAB chromogen.
References for Anti-Involucrin antibody [SY5], prediluted (ab74181)
ab74181 has not yet been referenced specifically in any publications.
