The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Ready to use. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37oC, or Protease XXV at 1mg/ml PBS for 5 minutes at 37oC.
Note: Enzyme digestion is better than citrate buffer based epitope unmasking.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPart of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Tissue specificityKeratinocytes of epidermis and other stratified squamous epithelia.
Sequence similaritiesBelongs to the involucrin family.
Post-translational modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
Cellular localizationCytoplasm. Constituent of the scaffolding of the cornified envelope.