Anti-Involucrin antibody (ab27495)
Key features and details
- Rabbit polyclonal to Involucrin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Involucrin antibody
See all Involucrin primary antibodies -
Description
Rabbit polyclonal to Involucrin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Involucrin aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: P07476 -
Positive control
- Human skin
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. -
Tissue specificity
Keratinocytes of epidermis and other stratified squamous epithelia. -
Sequence similarities
Belongs to the involucrin family. -
Post-translational
modificationsSubstrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. -
Cellular localization
Cytoplasm. Constituent of the scaffolding of the cornified envelope. - Information by UniProt
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Database links
- Entrez Gene: 3713 Human
- Omim: 147360 Human
- SwissProt: P07476 Human
- Unigene: 516439 Human
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Alternative names
- INVO_HUMAN antibody
- Involucrin antibody
- IVL antibody
Images
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab27495 has been referenced in 7 publications.
- Prabhakar AT et al. CK2 Phosphorylation of Human Papillomavirus 16 E2 on Serine 23 Promotes Interaction with TopBP1 and Is Critical for E2 Interaction with Mitotic Chromatin and the Viral Life Cycle. mBio 12:e0116321 (2021). PubMed: 34544280
- Hieda DS et al. Air Particulate Matter Induces Skin Barrier Dysfunction and Water Transport Alteration on a Reconstructed Human Epidermis Model. J Invest Dermatol N/A:N/A (2020). PubMed: 32339540
- Camarena DEM et al. Development of Epidermal Equivalent from Electrospun Synthetic Polymers for In Vitro Irritation/Corrosion Testing. Nanomaterials (Basel) 10:N/A (2020). PubMed: 33339410
- Evans MR et al. An oral keratinocyte life cycle model identifies novel host genome regulation by human papillomavirus 16 relevant to HPV positive head and neck cancer. Oncotarget 8:81892-81909 (2017). IHC-P ; Human . PubMed: 29137231
- Melo SP et al. Somatic correction of junctional epidermolysis bullosa by a highly recombinogenic AAV variant. Mol Ther 22:725-33 (2014). ICC/IF ; Human . PubMed: 24390279
- Lamb R & Ambler CA Keratinocytes propagated in serum-free, feeder-free culture conditions fail to form stratified epidermis in a reconstituted skin model. PLoS One 8:e52494 (2013). ICC/IF ; Human . PubMed: 23326335
- Brohem CA et al. Proteasome inhibition and ROS generation by 4-nerolidylcatechol induces melanoma cell death. Pigment Cell Melanoma Res 25:354-69 (2012). PubMed: 22372875