Overview

  • Product nameIonomycin (free acid)
  • Description
    Ca2+ ionophore
  • Biological descriptionPotent, highly selective Ca2+ ionophore. Effective mobile ion carrier for Ca2+, highly specific for divalent cations (Ca > Mg >> Sr = Ba). Useful for studies of Ca2+ transport across biological membranes and increasing intracellular Ca2+ concentrations. Complexes with Ca2+ between pH 7 and 9.5. Absorption max. (280 nm, MeOH): 18,600 M-1cm-1.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120370 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Ionomycin (free acid) images

    • ab58668 staining ATF3 in A549 cells treated with ionomycin (free acid) (ab120370), by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin (free acid), as described in literature.
      The cells were incubated at 37°C for 2h in media containing different concentrations of ab120370 (Ionomycin (free acid)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Ionomycin (free acid) (ab120370)

    This product has been referenced in:

    See all 2 Publications for this product

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    Product ab120116 (Ionomycin Ca2+ Salt) differs from ab120370 (free acid), as the presence of the Ca2+ makes the compound easier to solubilise (the free acid is soluble in ethanol to 10 mM and in DMSO to 10 mM. The...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"