![Western blot - GluR2 antibody [EP966Y] (ab52932) image](#ab52932_1.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Overview
Product name
Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y]
See all Ionotropic Glutamate receptor 2 products (8) ...
Description
Rabbit monoclonal [EP966Y] to Ionotropic Glutamate receptor 2
Tested applications
WB, IP, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues near the N terminus of human GluR2.
Positive control
Human brain cell lysate.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 0.87% Sodium chloride, 0.09% Tris glycine
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP966Y
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Glutamate Receptors
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
-
Western blot - GluR2 antibody [EP966Y] (ab52932)
![Western blot - GluR2 antibody [EP966Y] (ab52932) image](/ps/datasheet/Images/52/ab52932/ab52932_1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
IP
IP: 1/40IP: 1/40
Flow Cyt
Flow Cyt: 1/30Flow Cyt: 1/30
Application notes
Is unsuitable for ,ICC/IF,IHC-Fr or IHC-P.
Target
Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
Post-translational
modifications
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
Target information above from: UniProt accessionP42262
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AMPA 2 antibody
- AMPA 2 antibody
- AMPA selective glutamate receptor 2 antibody
see all
Database links
- Entrez Gene: 2891 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- SwissProt: P42262 Human
- SwissProt: P23819 Mouse
- SwissProt: P19491 Rat
- Unigene: 32763 Human
see all
Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] images:
Western blot - GluR2 antibody [EP966Y] (ab52932)
Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/2000 dilution + Human brain cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size : 99 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
References for Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)
ab52932 has not yet been referenced specifically in any publications.
Publishing research using ab52932? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"