Anti-Ionotropic Glutamate receptor 2 antibody (ab20673)

Western blot - GluR2 antibody (ab20673)

Lane 1 : Marker
Lanes 2 - 5 : Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml

Lane 1 : As above
Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
Lane 3 : Cortex (Mouse) Tissue Lysate at 10 µg
Lane 4 : Brain (Mouse) Tissue Lysate at 10 µg with GluR2 peptide (ab25708) at 1 µg/ml
Lane 5 : Cortex (Mouse) Tissue Lysate at 10 µg with GluR2 peptide (ab25708) at 1 µg/ml

Secondary
Lanes 2 - 5 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution


Performed under reducing conditions.

Predicted band size : 99 kDa
Observed band size : 99 kDa


Additional bands: The band observed at ~50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor. We are unsure as to the identity of the band at ~43KDa.

Western blot - GluR2 antibody (ab20673)

Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size : 99 kDa
Observed band size : 99 kDa
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.

The band observed at 50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor.

Immunohistochemistry (PFA perfusion fixed frozen sections) - Ionotropic Glutamate receptor 2 antibody (ab20673)

Immunohistochemistical detection of Ionotropic Glutamate receptor 2 using antibody (ab20673) on PFA perfusion-fixed frozen rat brain sections. Primary antibody was used at 1/300, incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). The image demonstrates immunostaining obtained in the rat cortex, thin and punctuated cytoplasmic staining is observed as previously described for the hippocampus (J neurosci 2003 Nov 1923(33):10521-30). There is some staining in cellular processes as well (arrows).

Dr Sophie Pezet, CNRS, Paris, France