Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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ab25708 |
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ab25708 |
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I have a question about ab20673, Ionotropic Glutamate receptor 2 antibody. What is the sequential homology between ab20673 and human GluR1 (SwissProt P42261), human GluR2 (SwissProt P42262), human GluR3 (SwissProt P42263) and human GluR4 (SwissProt P48058)? Has ab20673 tested reactivity with those human Glutamate receptors? |
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ANSWER: |
Thanks for your enquiry. The sequence homology between the immunogen sequence of ab20673 and human Glutamate receptors are as follows. human GluR1: 60% human GluR2: 92% human GluR3: 30% human GluR4: 40% Based on sequence analysis, ab20673 is most likely not reactive with other glutamate receptors (1, 3, 4). I hope this information helps you, please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lane 1 : Marker
Lanes 2 - 5 : Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml
Lane 1 : As above
Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
Lane 3 : Cortex (Mouse) Tissue Lysate at 10 µg
Lane 4 : Brain (Mouse) Tissue Lysate at 10 µg with
Lane 5 : Cortex (Mouse) Tissue Lysate at 10 µg with
Secondary
Lanes 2 - 5 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 99 kDa
Observed band size : 99 kDa
Additional bands: The band observed at ~50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor. We are unsure as to the identity of the band at ~43KDa.
Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 99 kDa
Observed band size : 99 kDa
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
The band observed at 50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor.
Immunohistochemistical detection of Ionotropic Glutamate receptor 2 using antibody (ab20673) on PFA perfusion-fixed frozen rat brain sections. Primary antibody was used at 1/300, incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). The image demonstrates immunostaining obtained in the rat cortex, thin and punctuated cytoplasmic staining is observed as previously described for the hippocampus (J neurosci 2003 Nov 1923(33):10521-30). There is some staining in cellular processes as well (arrows).
Dr Sophie Pezet, CNRS, Paris, France
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