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Western blot - GluR2 antibody (ab20673)
(enlarge)
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Western blot - GluR2 antibody (ab20673)
(enlarge)
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Product Name
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Ionotropic Glutamate receptor 2 antibody
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See all Ionotropic Glutamate receptor 2 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Ionotropic Glutamate receptor 2
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GluR2 .
(Peptide available as ab25708.)
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Reacts with
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Ms, Rat
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Predicted to react
(species key)
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Hu, Chk
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 3-10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). Can be blocked with GluR2 peptide (ab25708).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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ab20673 gave a positive result in the following tissue lysates:
Mouse brain
Mouse cortex
Rat brain
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Cellular localization
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Cell Membrane
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Research areas
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Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Glutamate Receptors
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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Relevance
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Glutamate receptors sensitive to AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolpropionate) are ligand-activated cation channels that mediate the fast component of excitatory postsynaptic currents in neurons of the central nervous system. These channels are assembled from 4 related subunits, GLURA (GRIA1), GLURB (GRIA2), GLURC (GRIA3), GLURD (GRIA4), with the GLURB subunit rendering the channel almost impermeable to Ca(2+).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Ionotropic Glutamate receptor 2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Ionotropic Glutamate receptor 2 antibody images, references, products related to ab20673 and other tools.
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Ionotropic Glutamate receptor 2 antibody images:
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 Western blot - GluR2 antibody (ab20673)
Lane 1 : Marker
Lanes 2 - 5 : Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml
Lane 1 : As above
Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
Lane 3 : Cortex (Mouse) Tissue Lysate at 10 µg
Lane 4 : Brain (Mouse) Tissue Lysate at 10 µg with GluR2 peptide (ab25708) at 1 µg/ml
Lane 5 : Cortex (Mouse) Tissue Lysate at 10 µg with GluR2 peptide (ab25708) at 1 µg/ml
Secondary
Lanes 2 - 5 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 99 kDa
Observed band size : 99 kDa
Additional bands: The band observed at ~50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor. We are unsure as to the identity of the band at ~43KDa.
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Western blot - GluR2 antibody (ab20673)
Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 99 kDa
Observed band size : 99 kDa
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
The band observed at 50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor.
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References for Ionotropic Glutamate receptor 2 antibody (ab20673)
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This product has been used in:
M nard C et al. AMPA receptor-mediated cell death is reduced by docosahexaenoic acid but not by eicosapentaenoic acid in area CA1 of hippocampal slice cultures. J Neurosci Res 87:876-86 (2009). WB; Rat. PubMed: 18951489
If you publish research using ab20673 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Ionotropic Glutamate receptor 2
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Ionotropic Glutamate receptor 2 antibody - more information
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