Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Overview
Product name
Anti-Ionotropic Glutamate receptor 2 antibody
See all Ionotropic Glutamate receptor 2 products (8) ...
Description
Rabbit polyclonal to Ionotropic Glutamate receptor 2
Tested applications
IHC-FoFr, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Chicken, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GluR2 .
(Peptide available as ab25708.)
Positive control
ab20673 gave a positive result in the following tissue lysates: Mouse brain Mouse cortex Rat brain
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Glutamate Receptors
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: 1/300
WB: Use a concentration of 3-10 µg/mlDetects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).Can be blocked with GluR2 peptide (ab25708).
Target
Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
Post-translational
modifications
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
Target information above from: UniProt accessionP42262
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AMPA 2 antibody
- AMPA 2 antibody
- AMPA selective glutamate receptor 2 antibody
see all
Database links
- Entrez Gene: 414894 Chicken
- Entrez Gene: 2891 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- SwissProt: P42262 Human
- SwissProt: P23819 Mouse
- SwissProt: P19491 Rat
see all
Anti-Ionotropic Glutamate receptor 2 antibody images:
Western blot - GluR2 antibody (ab20673)
Lane 1 : Marker
Lanes 2 - 5 : Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml
Lane 1 : As above
Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
Lane 3 : Cortex (Mouse) Tissue Lysate at 10 µg
Lane 4 : Brain (Mouse) Tissue Lysate at 10 µg with GluR2 peptide (ab25708) at 1 µg/ml
Lane 5 : Cortex (Mouse) Tissue Lysate at 10 µg with GluR2 peptide (ab25708) at 1 µg/ml
Secondary
Lanes 2 - 5 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 99 kDa
Observed band size : 99 kDa
Additional bands: The band observed at ~50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor. We are unsure as to the identity of the band at ~43KDa.
Western blot - GluR2 antibody (ab20673)
Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 99 kDa
Observed band size : 99 kDa
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
The band observed at 50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Ionotropic Glutamate receptor 2 antibody (ab20673)
Immunohistochemistical detection of Ionotropic Glutamate receptor 2 using antibody (ab20673) on PFA perfusion-fixed frozen rat brain sections. Primary antibody was used at 1/300, incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). The image demonstrates immunostaining obtained in the rat cortex, thin and punctuated cytoplasmic staining is observed as previously described for the hippocampus (J neurosci 2003 Nov 1923(33):10521-30). There is some staining in cellular processes as well (arrows).
Dr Sophie Pezet, CNRS, Paris, France
References for Anti-Ionotropic Glutamate receptor 2 antibody (ab20673)
This product has been referenced in:
- Ménard Cet al. AMPA receptor-mediated cell death is reduced by docosahexaenoic acid but not by eicosapentaenoic acid in area CA1 of hippocampal slice cultures. J Neurosci Res 87:876-86 (2009). WB; Rat.Read more (PubMed: 18951489) »
See 1 publication for this product
Publishing research using ab20673? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"