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Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Overview
Product name
Anti-Ionotropic Glutamate receptor 2 (phospho S880) antibody
See all Ionotropic Glutamate receptor 2 products (8) ...
Description
Rabbit polyclonal to Ionotropic Glutamate receptor 2 (phospho S880)
Specificity
Detects endogenous levels of Glutamate receptor 2 only when phosphorylated at serine 880.
Tested applications
ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human Glutamate receptor 2 around the phosphorylation site of serine 880 (I-E-SP-V-K).
Positive control
Extracts from mouse brain, human brain tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Glutamate Receptors
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000.Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
Target
Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
Post-translational
modifications
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
Target information above from: UniProt accessionP42262
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AMPA 2 antibody
- AMPA 2 antibody
- AMPA selective glutamate receptor 2 antibody
see all
Database links
- Entrez Gene: 2891 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- SwissProt: P42262 Human
- SwissProt: P23819 Mouse
- SwissProt: P19491 Rat
- Unigene: 32763 Human
see all
Anti-Ionotropic Glutamate receptor 2 (phospho S880) antibody images:
Western blot - GluR2 (phospho S880) antibody (ab52180)
All lanes : Anti-Ionotropic Glutamate receptor 2 (phospho S880) antibody (ab52180) at 1/500 dilution
Lane 1 : extracts from mouse brain
Lane 2 : extracts from mouse brain with blocking phosphopeptide
Predicted band size : 99 kDa
Immunohistochemistry (Paraffin-embedded sections) - GluR2 (phospho S880) antibody (ab52180)
Ab52180 at 1/50 dilution staining Human brain tissue, with and without blocking peptide; paraffin embedded.
References for Anti-Ionotropic Glutamate receptor 2 (phospho S880) antibody (ab52180)
This product has been referenced in:
- Miñano-Molina AJet al. Soluble Oligomers of Amyloid-{beta} Peptide Disrupt Membrane Trafficking of {alpha}-Amino-3-hydroxy-5-methylisoxazole-4-propionic Acid Receptor Contributing to Early Synapse Dysfunction. J Biol Chem 286:27311-21 (2011). WB; Mouse.Read more (PubMed: 21665950) »
- Gu Zet al. {beta}-Amyloid Impairs AMPA Receptor Trafficking and Function by Reducing Ca2+/Calmodulin-dependent Protein Kinase II Synaptic Distribution. J Biol Chem 284:10639-49 (2009). WB; Mouse.Read more (PubMed: 19240035) »
See all 2 publications for this product
Publishing research using ab52180? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"