Anti-Ionotropic Glutamate receptor 4 antibody [EPR2512(2)] (ab109431)

Overview

• Product nameAnti-Ionotropic Glutamate receptor 4 antibody [EPR2512(2)]See all Ionotropic Glutamate receptor 4 primary antibodies ...
• Description
  Rabbit monoclonal [EPR2512(2)] to Ionotropic Glutamate receptor 4
• Tested applicationsWB, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues in the intracellular domain in Human Ionotropic Glutamate receptor 4.

• Positive controlRat brain, Mouse brain, Human cerebellum, and SH-SY5Y lysates; SH-SY5Y cells
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR2512(2)
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • GPCR

  • Neuroscience
  • Neurotransmission
  • Receptors / Channels
  • GPCR
  • Glutamate Receptors

Applications

Target

• FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
• Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily.
• Post-translational
  modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
• Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.

Target information above from: UniProt accession P48058 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2893 Human
  • Entrez Gene: 14802 Mouse
  • Entrez Gene: 29629 Rat
  • Omim: 138246 Human
  • SwissProt: P48058 Human
  • SwissProt: Q9Z2W8 Mouse
  • SwissProt: P19493 Rat
  • Unigene: 503743 Human

  • Unigene: 209263 Mouse
  • Unigene: 10938 Rat

  see all

• Alternative names
  AMPA 4 antibodyAMPA selective glutamate receptor 4 antibodyAMPA-selective glutamate receptor 4 antibody

  AMPA4 antibodyGluA 4 antibodyGluA4 antibodyGluR 4 antibodyGluR D antibodyGluR-4 antibodyGluR-D antibodyGluR4 antibodyGLUR4C antibodyGLURD antibodyGlutamate receptor 4 antibodyGlutamate receptor ionotrophic AMPA 4 antibodyGlutamate receptor ionotropic antibodyGRIA 4 antibodyGria4 antibodyGRIA4_HUMAN antibody

  see all

Anti-Ionotropic Glutamate receptor 4 antibody [EPR2512(2)] images

• 

  Western blot - Ionotropic Glutamate receptor 4 antibody [EPR2512(2)] (ab109431)
  All lanes : Anti-Ionotropic Glutamate receptor 4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution
  
  Lane 1 : Rat brain lysate
  Lane 2 : Mouse brain lysate
  Lane 3 : Human cerebellum lysate
  Lane 4 : SH-SY5Y lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 101 kDa
  
• 

  Flow Cytometry - Ionotropic Glutamate receptor 4 antibody [EPR2512(2)] (ab109431)
  Flow cytometric analysis of permeabilized SH-SY5Y cells using ab109431 at 1/10 dilution (red) or a rabbit IgG (negative) (green).