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Overview

  • Product nameIron Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 8 µM
  • Range
    8 µM - 400 µM
  • Assay time
    1h 00m
  • Product overview

    Abcam's Iron Assay Kit provides a simple convenient means of measuring Ferrous and/or Ferric ion in sample. In the assay, ferric carrier protein will dissociate ferric into solution in the presence of acid buffer. After reduction to the ferrous form (Fe2+), iron reacts with Ferene S to produce a stable colored complex and give absorbance at 593 nm. A specific chelate chemical is included in the buffer to block copper ion (Cu2+) interference. The kit measures iron in the linear range of 0.4 to 20 nmol in 50 µl sample, or 8 µM to 400 µM iron concentration in various samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Iron is essential to nearly all known organisms. It is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Inorganic iron also contributes to redox reactions in the iron-sulfur clusters of many enzymes, such as nitrogenase and hydrogenase.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceIron is essential to nearly all known organisms. It is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Inorganic iron also contributes to redox reactions in the iron-sulfur clusters of many enzymes, such as nitrogenase and hydrogenase.
    • Alternative names
        FeFe++Fe+++

    Applications

    Our Abpromise guarantee covers the use of ab83366 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Iron Assay Kit images

    • Functional Studies - Iron Assay Kit (ab83366)
      Functional Studies - Iron Assay Kit (ab83366)
      Example of iron standard curve using ab83366
    • Functional Studies - Iron Assay Kit (ab83366)
      Functional Studies - Iron Assay Kit (ab83366)
      Example of a Sample time line using ab83366

    Protocols

    References for Iron Assay Kit (ab83366)

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab83366 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab83366 Submit a Question for ab83366

    Displaying 1 - 10 of 24 results for Abreviews and Q&A

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    Just want to know what kind of compounds are in the iron assay buffer? I need to use the same samples for measuring both iron and proteins, and I'm using the folin method to measure proteins. Am I able to use the same cells lysed with iron assay buffer to...

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    Unfortunately, the assay buffer composition is considered as proprietary information. However, I can confirm that it is compatible with the mentioned method to measure proteins.

    To order from New Zealand, please contact our authorised ...

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    i want to measure ferrous and ferric acid separately, can I do that with this kit?

    Thank you for contacting us.


    Yes, you can definitely measure ferrous and ferric acid separately with this kit. Please follow the protocol for measuring the ferrous and the total iron in the samples. When you subtract the ferrous levels fro...

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    Customer wants to know:
    1) Can one test ferrous and ferric ions separately?
    2) Can one use heat denatured muscle tissue for the sample?
    3) Is Fe3 stable throughout the process?

    Thank you for your enquiry.

    Question 1: Can one test ferrous and ferric ions separately?

    Answer 1: This test can for ferrous and ferric ions separately.

    Question 2: Can one use heat denatured muscle tissue for the sample? Read More

    Dear Supplier,

    Our customer would like to find out if following kits are suitable for his use. He would like to measure ATP, chelatable iron and glycogen in rat liver tissues and chelatable iron in rat liver perfusate samples.:

    ab833...

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    Thank you for your enquiry.

    I can confirm you can use perfused liver samples with all these kits.

    ab83366 Iron Assay Kit will detect chelatable iron.

    The kits are suitable for all mammalian species.

    I hope this ...

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    Kindly advise if Iron Assay Buffer and Iron Probe can be purchased separately from ab83366.
    Thanks in advance fro to your assistance.
    Have a nice day.

    Thank you for contacting us.
    These products are only available for sale as part of the kit only. We do not sell these products as individual components.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need ...

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    Dear abcam support, We'd like to measure iron with cells (ex: mouse embryonic fibroblasts). Before we buy this kit, I borrowed this kit and tested. As your protocol, I lysed cells (2 x 106) with 500 ul of Iron Assay Buffer, centrifuged and measured iron l...

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    We have received some additional information from the lab regarding ab83366. This assay works in an acidic environment. That is why when you add the HCl the samples are turning clear. One of the reasons that the samples are not changing color is becaus...

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    Product code: 83366
    Lot number: GR65506-2

    Inquiry: According to the kit protocol we tried the standard iron curve but we didnt got it. According to the protocol 100mM of Iron standard was diluted into a 96-well plate to generate 0, 2, 4, 6...

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    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this kit.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful ...

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    Our customer is going to purchase ab83366 and would like to know the expiration date of this product.



    We do not have set expiration dates for our products.
    The kit ab83366 is stable for 12 months at -20°C upon delivery.

    I hope to buy your product :Iron Assay Kit (ab83366) .

    But i don't know what kind of species it reactivity with? mice ,rat , or others?

    Thank you for contacting us.

    This kit is specific for mammals. You could use it with both mouse and rat samples.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informatio...

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    How much volume of cells should be used with tis kit.
    Do we need to pellet the cells or cells in supension will be fine.

    Thank you for contacting us.

    For cell samples: Start with ˜2X10e6 cells, suspend the cell pellet 500 μl (or ˜4 volumes) of the assay buffer on ice, homogenize using a Dounce homogenizer (10-50 passes) on ice, until efficient lysis is confirm...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"