Anti-Iroquois homeobox protein 3/IRX3 antibody (ab25703)
Key features and details
- Rabbit polyclonal to Iroquois homeobox protein 3/IRX3
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Iroquois homeobox protein 3/IRX3 antibody
See all Iroquois homeobox protein 3/IRX3 primary antibodies -
Description
Rabbit polyclonal to Iroquois homeobox protein 3/IRX3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Chicken, Human, Xenopus laevis, Zebrafish -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 52, 55 kDa (predicted molecular weight: 52 kDa).
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Notes |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 52, 55 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells. -
Sequence similarities
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 408181 Chicken
- Entrez Gene: 79191 Human
- Entrez Gene: 16373 Mouse
- Entrez Gene: 399365 Xenopus laevis
- Entrez Gene: 30520 Zebrafish
- Omim: 612985 Human
- SwissProt: P78415 Human
- SwissProt: P81067 Mouse
see all -
Alternative names
- AI894186 antibody
- Homeodomain protein IRXB1 antibody
- Iroquois class homeodomain protein IRX 1 antibody
see all
Images
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All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody (ab25703) at 2 µg/ml
Lane 1 : Mouse Fetus (14 Day Old) Tissue Lysate
Lane 2 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52,55 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab25703 has been referenced in 6 publications.
- Bjune JI et al. The homeobox factor Irx3 maintains adipogenic identity. Metabolism 103:154014 (2020). PubMed: 31751577
- Tamamura Y et al. Irx3 and Bmp2 regulate mouse mesenchymal cell chondrogenic differentiation in both a Sox9-dependent and -independent manner. J Cell Physiol 232:3317-3336 (2017). PubMed: 28059449
- Zou Y et al. IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity Risk. EBioMedicine 24:64-75 (2017). PubMed: 28988979
- Wang P et al. Downregulation of miR-377 contributes to IRX3 deregulation in hepatocellular carcinoma. Oncol Rep 36:247-52 (2016). PubMed: 27222047
- Shekhar A et al. Transcription factor ETV1 is essential for rapid conduction in the heart. J Clin Invest 126:4444-4459 (2016). IHC-Fr ; Mouse . PubMed: 27775552
- Gaborit N et al. Cooperative and antagonistic roles for Irx3 and Irx5 in cardiac morphogenesis and postnatal physiology. Development 139:4007-19 (2012). ChIP ; Mouse . PubMed: 22992950