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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Overview
Product name
Anti-Islet 1 antibody - Neural Stem Cell Marker
See all Islet 1 products (6) ...
Description
Rabbit polyclonal to Islet 1 - Neural Stem Cell Marker
Specificity
ab20670 might also detect rat and human Islet 2 protein as the immunogen used to raise this ab20670 is 86% identical to rat and human Islet 2. This has not been tested.
Tested applications
IHC-FoFr, IF, IHC-P, ICC/IF, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Apteronotus leptorhynchus
Predicted to work with
Chicken, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Islet 1.
(Peptide available as ab21996.)
Positive control
4 day old cultures of rat dorsal root ganglion neurons (16 day old rat embryos).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Gut development >> Pancreas development
Developmental Biology >> Lineage specification >> Endoderm
Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cardiovascular >> Atherosclerosis >> Diabetes associated
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Endoderm
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 20096094)
IF: Use at an assay dependent dilution.
IHC-P: Use a concentration of 2 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 2 µg/ml
IHC-Fr: 1/500
Target
Function
Binds to one of the cis-acting domain of the insulin gene enhancer.
Tissue specificity
Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP61371
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Insulin gene enhancer protein ISL 1 antibody
- Insulin gene enhancer protein ISL-1 antibody
- Insulin related protein antibody
see all
Database links
- Entrez Gene: 3670 Human
- Entrez Gene: 16392 Mouse
- Entrez Gene: 64444 Rat
- Entrez Gene: 30147 Zebrafish
- Omim: 600366 Human
- SwissProt: P50211 Chicken
- SwissProt: P61371 Human
- SwissProt: P61372 Mouse
see all
Anti-Islet 1 antibody - Neural Stem Cell Marker images:
Immunocytochemistry/ Immunofluorescence
Dorsal root ganglion explants were dissected from 16 day-old rat embryos and cultured for 6 hours in vitro with Neurobasal Medium containing B27 supplement.
Nuclei stained positive for anti-Islet 1 antibody ab20670 at 2µg/ml. As would be expected, not all cells in this preparation were Islet 1-positive. Pre-incubation of ab20670 with the immunizing peptide ab21996 resulted in complete blocking of the antibody.
Green = ab20670
Blue = To-pro-3 nuclear stain
The level of magnification is different in each image.
Randal Moldrich, CNRS UMR7637, ESPCI, France
Immunohistochemistry (Frozen sections) - Islet 1 antibody - Neural Stem Cell Marker (ab20670)
ab20670 detected Islet 1 in the nucleus of frozen sections of E11.5 mouse embryonic brain using 1 ug/ml of antibody. Brighter staining may be achieved using a greater concentration of antibody, e.g. 2.5 or 5 ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Islet 1 antibody - Neural Stem Cell Marker(ab20670)
ab20670 2µg/ml staining ISLET1 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and weak cytoplasmic staining primarily in the pancreatic islet.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-Islet 1 antibody - Neural Stem Cell Marker (ab20670)
This product has been referenced in:
- Dykes IMet al. Brn3a and islet1 act epistatically to regulate the gene expression program of sensory differentiation. J Neurosci 31:9789-99 (2011).Read more (PubMed: 21734270) »
- Ma Tet al. A Subpopulation of Dorsal Lateral/Caudal Ganglionic Eminence-Derived Neocortical Interneurons Expresses the Transcription Factor Sp8. Cereb Cortex : (2011).Read more (PubMed: 22021915) »
See all 7 publications for this product
Publishing research using ab20670? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"