Islet 1 antibody - Neural Stem Cell Marker (ab26122)

Product Name 

Islet 1 antibody - Neural Stem Cell Marker

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Islet 1 - Neural Stem Cell Marker

Immunogen 

Recombinant fusion protein from Islet 1 sequence C-terminal to the homeodomain (Rat)

Reacts with 

Ms, Chk

Predicted to react 
(species key)

Hu, Rat
(Due to sequence homology)

Specificity 

ab26122 may cross react with Islet 2 protein.

Tested applications 
(see key)

ICC, IHC (PFA fixed)

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: 1/5000.
Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse Embryonic Spinal Cord (E9.5-E15.5)
Mouse Embryonic DRG

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Organogenesis >> Gut development >> Pancreas development
Developmental Biology >> Lineage specification >> Endoderm
Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Endoderm
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker

Relevance 

Islet 1 is a transcription factor containing two amino-terminal LIM domains and one carboxy-terminal homeodomain. Islet 1 plays an important role in the embryogenesis of pancreatic islets of Langerhans. In addition, mouse embryos made deficient in Islet 1 fail to undergo neural tube motor neuron differentiation.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.

References for Islet 1 antibody - Neural Stem Cell Marker (ab26122)

  This product has been used in: (two most recent references)

Geisen MJ et al. Hox paralog group 2 genes control the migration of mouse pontine neurons through slit-robo signaling. PLoS Biol 6:e142 (2008). IHC-FoFr; Mouse. PubMed: 18547144

Boije H et al. Temporal and spatial expression of transcription factors FoxN4, Ptf1a, Prox1, Isl1 and Lim1 mRNA in the developing chick retina. Gene Expr Patterns 8:117-23 (2008). PubMed: 18006384
If you publish research using ab26122 please let us know so that we can cite the reference on this datasheet.

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