Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Overview
Product name
Anti-Islet 1 antibody - Neural Stem Cell Marker
See all Islet 1 products (6) ...
Description
Rabbit polyclonal to Islet 1 - Neural Stem Cell Marker
Specificity
ab26122 may cross react with Islet 2 protein.
Tested applications
Sandwich ELISA, ICC, IHC (PFA fixed), IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Chicken
Predicted to work with
Rat, Human
Immunogen
Recombinant fusion protein from Islet 1 sequence C-terminal to the homeodomain (Rat)
Positive control
Mouse Embryonic Spinal Cord (E9.5-E15.5) Mouse Embryonic DRG
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Gut development >> Pancreas development
Developmental Biology >> Lineage specification >> Endoderm
Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cardiovascular >> Atherosclerosis >> Diabetes associated
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Endoderm
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use a concentration of 0.1 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal [1B1] to Islet 1 (ab86501). (For sandwich ELISA, use this antibody as Detection at 0.1 µg/ml with Mouse monoclonal [1B1] to Islet 1 (ab86501) as Capture.)
ICC
ICC: 1/5000ICC: 1/5000
IHC (PFA fixed): Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 21533046)
Target
Function
Binds to one of the cis-acting domain of the insulin gene enhancer.
Tissue specificity
Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP61371
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Insulin gene enhancer protein ISL 1 antibody
- Insulin gene enhancer protein ISL-1 antibody
- Insulin related protein antibody
see all
Database links
- Entrez Gene: 3670 Human
- Entrez Gene: 16392 Mouse
- Entrez Gene: 64444 Rat
- Omim: 600366 Human
- SwissProt: P50211 Chicken
- SwissProt: P61371 Human
- SwissProt: P61372 Mouse
- SwissProt: P61374 Rat
see all
Anti-Islet 1 antibody - Neural Stem Cell Marker images:
Sandwich ELISA - Islet 1 antibody - Neural Stem Cell Marker (ab26122)
Standard Curve for Islet 1; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1B1] to Islet 1 (ab86501) at 1 ug/ml and Detector Antibody Rabbit polyclonal to Islet 1 - Neural Stem Cell Marker (ab26122) at 0.1 ug/ml.
- Islet 1 antibody - Neural Stem Cell Marker (ab26122)
ab26122 staining Islet 1 in murine hindbrain tissue by Immunohistochemistry (PFA fixed).E15.5 WT hindbrain doubly immunostained using anti-Robo3 (green) and ab26122 (red) antibodies. After dissection, brains were fixed by immersion in 4% paraformaldehyde in 0.12 M phosphate buffer (pH 7.4) (PFA). Brains were blocked in 0.2% gelatin in PBS containing 0.25% Triton-X100 and incubated overnight at room temperature with goat anti-human ROBO3 and ab26122 followed by species-specific fluorochrome-coupled secondary antibodies donkey anti-goat Cy3 and donkey anti-goat A488.PN - Pontine neurons.FMN - Facial motor nucleus.
Image from Geisen MJ et al, PLoS Biol. 2008 Jun 10;6(6):e142, Fig 4.
Protocols
References for Anti-Islet 1 antibody - Neural Stem Cell Marker (ab26122)
This product has been referenced in:
- Ackermann Jet al. Loss of ATF2 Function Leads to Cranial Motoneuron Degeneration during Embryonic Mouse Development. PLoS One 6:e19090 (2011). IHC-Fr; Mouse.Read more (PubMed: 21533046) »
- Geisen MJet al. Hox paralog group 2 genes control the migration of mouse pontine neurons through slit-robo signaling. PLoS Biol 6:e142 (2008). IHC-FoFr; Mouse.Read more (PubMed: 18547144) »
See all 3 publications for this product
Publishing research using ab26122? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"