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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Overview
Product name
Anti-Islet 1 antibody
See all Islet 1 products (6) ...
Description
Rabbit polyclonal to Islet 1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Cow
Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 108-157 of human Islet 1 (NP_002193)
VACSRQLIPG DEFALREDGL FCRADHDVVE RASLGAGDPL SPLHPARPLQ
Positive control
Fetal muscle lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified by peptide affinity chromatography method.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Gut development >> Pancreas development
Developmental Biology >> Lineage specification >> Endoderm
Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cardiovascular >> Atherosclerosis >> Diabetes associated
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Endoderm
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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Western blot - Islet 1 antibody (ab80556)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 39 kDa.
Target
Function
Binds to one of the cis-acting domain of the insulin gene enhancer.
Tissue specificity
Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP61371
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Insulin gene enhancer protein ISL 1 antibody
- Insulin gene enhancer protein ISL-1 antibody
- Insulin related protein antibody
see all
Database links
- Entrez Gene: 3670 Human
- Entrez Gene: 16392 Mouse
- Entrez Gene: 64444 Rat
- Omim: 600366 Human
- SwissProt: P61371 Human
- SwissProt: P61372 Mouse
- SwissProt: P61374 Rat
- Unigene: 505 Human
see all
Anti-Islet 1 antibody images:
Western blot - Islet 1 antibody (ab80556)
Anti-Islet 1 antibody (ab80556) at 1 µg/ml + fetal muscle lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 39 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
References for Anti-Islet 1 antibody (ab80556)
ab80556 has not yet been referenced specifically in any publications.
Publishing research using ab80556? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"