Overview

  • Product nameIsocitrate Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.2 µg
  • Range
    0.2 µg - 5 µg
  • Assay time
    0h 40m
  • Product overview

    Abcam's Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In the assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a λmax of 450nm. The Isocitrate Assay Kit can detect 1 to 20 nmoles (~0.2-5 µg) of isocitrate.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Isocitric acid (HOOC-CHOH-CH (-COOH)-CH2-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and α-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceIsocitric acid (HOOC-CHOH-CH (-COOH)-CH2-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and alpha-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to alpha-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa.
  • Alternative names
    • Isocitric acid

Applications

Our Abpromise guarantee covers the use of ab83424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution.

Isocitrate Assay Kit images

  • Isocitrate standard curve generated using this kit protocol.

Protocols

References for Isocitrate Assay Kit (ab83424)

ab83424 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I would be pleased to help answer your questions. 1. I can confirm you should be able to use these kits with blood samples. Generally serum or plasma that is obtained from blood samples is used for such purposes. Howev...

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Thank you for your enquiry regarding ab83396 and ab83424. Yes, you can use these kits with blood samples. I would suggest obtaining serum / plasma from the blood sample before proceeding with the assay. For blood plasma- “Blood plasma is prepared by...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"