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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
Abcam's Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In the assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a λmax of 450nm. The Isocitrate Assay Kit can detect 1 to 20 nmoles (~0.2-5 µg) of isocitrate.
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Isocitric acid (HOOC-CHOH-CH (-COOH)-CH2-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and α-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa.
|Isocitrate Assay Buffer||WM||1 x 25ml|
|Isocitrate Enzyme Mix||Green||1 x 200µl|
|Isocitrate Standard (100 mM) (Lyophilised)||Yellow||1 vial|
|Substrate Mix (Lyophilised)||Purple||1 vial|
Our Abpromise guarantee covers the use of ab83424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
ab83424 has not yet been referenced specifically in any publications.
Publishing research using ab83424 ? Please let us know so that we can cite the reference in this datasheet
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