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Isocitrate Assay Kit (ab83424) provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In this assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a λmax of 450nm. This assay can detect 1 to 20 nmoles (~0.2-5 µg) of isocitrate.
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Isocitric acid (HOOC-CHOH-CH (-COOH)-CH2-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and α-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa.
|Isocitrate Assay Buffer||WM||1 x 25ml|
|Isocitrate Enzyme Mix||Green||1 x 200µl|
|Isocitrate Standard (100 mM) (Lyophilised)||Yellow||1 vial|
|Substrate Mix (Lyophilised)||Purple||1 vial|
Our Abpromise guarantee covers the use of ab83424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
ab83424 has not yet been referenced specifically in any publications.