Isocitrate Assay Kit (ab83424)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Food samples, Tissue Lysate
- Sensitivity: 0.2 µg
Overview
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Product name
Isocitrate Assay Kit -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Lysate, Food samples -
Assay type
Quantitative -
Sensitivity
> 0.2 µg -
Range
0.2 µg - 5 µg -
Assay time
0h 40m -
Product overview
Isocitrate Assay Kit (ab83424) provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In this assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a λmax of 450nm. This assay can detect 1 to 20 nmoles (~0.2-5 µg) of isocitrate.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K656 Isocitrate Colorimetric Assay Kit. K656-100 is the same size as the 100 test size of ab83424.
Isocitric acid (HOOC-CHOH-CH (-COOH)-CH2-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and α-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Developer (Lyophilised) 1 vial Isocitrate Assay Buffer 1 x 25ml Isocitrate Enzyme Mix 1 x 200µl Isocitrate Standard 1 x 100µl -
Research areas
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Relevance
Isocitric acid (HOOC-CHOH-CH (-COOH)-CH2-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and alpha-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to alpha-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa. -
Alternative names
- Isocitric acid
Associated products
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Related Products
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab83424 has not yet been referenced specifically in any publications.