|
|
|
|
ELISA - Isocitrate dehydrogenase antibody (ab36329)
(enlarge)
|
|
|
|
|
Product Name
|
Isocitrate dehydrogenase antibody
|
| |
See all Isocitrate dehydrogenase antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Isocitrate dehydrogenase
|
|
Immunogen
|
Full length native protein purified from porcine heart
|
|
Reacts with
(species key)
|
Ms
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/400 - 1/1600.
WB: 1/500 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
WB: mouse tissues including spleen, kidney, lung, heart, liver and brain.
|
|
Cellular localization
|
Cytoplasm. Peroxisome.
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Energy Metabolism
|
|
Relevance
|
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
|
|
|
Isocitrate dehydrogenase antibody (ab36329) |
|
Database links
|
The links below go to external sites and will open in a new browser window Entrez Gene | | 15926 (Mouse)
|
| |
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Ab36329 was purified by Ammonium Sulphate fractionation.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Isocitrate dehydrogenase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Isocitrate dehydrogenase antibody images, references, products related to ab36329 and other tools.
|
| |
Isocitrate dehydrogenase antibody images:
|
 ELISA - Isocitrate dehydrogenase antibody (ab36329)
Titration curve of ab36329, rabbit antibody to Isocitrate dehyrogenase (ICDH). Plates coated with 400 ng/well of native Isocitrate Dedydrogenease derived from pig heart.
|
| |
| |
|
Search PubMed (MEDLINE) for references to Isocitrate dehydrogenase
|
| |
Isocitrate dehydrogenase antibody - more information
|
Customer reviews (feedback) regarding Isocitrate dehydrogenase antibody |
Customer FAQs regarding Isocitrate dehydrogenase antibody |
Protocols for Isocitrate dehydrogenase antibody |
Price and availability of products related to Isocitrate dehydrogenase antibody |
| |
Isocitrate dehydrogenase antibody - related products:
|
|
|
| |
Isocitrate dehydrogenase antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Isocitrate dehydrogenase antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
