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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Isocitrate dehydrogenase antibody
See all Isocitrate dehydrogenase products (8) ...
Description
Rabbit polyclonal to Isocitrate dehydrogenase
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Full length native protein purified from porcine heart
Positive control
WB: mouse tissues including spleen, kidney, lung, heart, liver and brain.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purification notes
Ab36329 was purified by Ammonium Sulphate fractionation.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Energy Metabolism
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ELISA - Isocitrate dehydrogenase antibody (ab36329)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/400 - 1/1600.
WB: 1/500 - 1/2000.Detects a band of approximately 40 kDa (predicted molecular weight: 47 kDa).
Target
Involvement in disease
Defects in IDH1 are involved in the development of glioma (GLM) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. Elevated levels of R(-)-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Cellular localization
Cytoplasm. Peroxisome.
Target information above from: UniProt accessionO75874
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- ICDH antibody
see all
Database links
- Entrez Gene: 15926 Mouse
- SwissProt: O88844 Mouse
- Unigene: 9925 Mouse
Anti-Isocitrate dehydrogenase antibody images:
ELISA - Isocitrate dehydrogenase antibody (ab36329)
Titration curve of ab36329, rabbit antibody to Isocitrate dehyrogenase (ICDH). Plates coated with 400 ng/well of native Isocitrate Dedydrogenease derived from pig heart.
References for Anti-Isocitrate dehydrogenase antibody (ab36329)
This product has been referenced in:
- Mancone Cet al. Proteomic analysis reveals a major role for contact inhibition in the terminal differentiation of hepatocytes. J Hepatol 52:234-43 (2010). WB; Mouse.Read more (PubMed: 20031246) »
- Mailloux RJ & Harper ME Glucose regulates enzymatic sources of mitochondrial NADPH in skeletal muscle cells; a novel role for glucose-6-phosphate dehydrogenase. FASEB J 24:2495-506 (2010). WB; Mouse.Read more (PubMed: 20228249) »
See all 4 publications for this product
Publishing research using ab36329? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"