You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Isocitrate dehydrogenase antibody (ab81653)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Isocitrate dehydrogenase antibody
See all Isocitrate dehydrogenase products (8) ...

Description

Rabbit polyclonal to Isocitrate dehydrogenase

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Sheep, Cow, Cat, Dog, Pig, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster)

Immunogen

Synthetic peptide from within residues: LGMMTSVLVC PDGKTVEAEA AHGTVTRHYR MYQKGQETST NPIASIFAWT, corresponding to internal sequence amino acids 288-337 of Human Isocitrate dehydrogenase (NP_005887)

Positive control

721_B cell lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Isocitrate dehydrogenase antibody (ab81653)Western blot - Isocitrate dehydrogenase antibody (ab81653) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isocitrate dehydrogenase antibody (ab81653)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isocitrate dehydrogenase antibody (ab81653) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isocitrate dehydrogenase antibody (ab81653)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isocitrate dehydrogenase antibody (ab81653) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab81653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Involvement in disease

Defects in IDH1 are involved in the development of glioma (GLM) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. Elevated levels of R(-)-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Cellular localization

Cytoplasm. Peroxisome.

Target information above from: UniProt accessionO75874 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cytosolic NADP isocitrate dehydrogenase antibody
  • Cytosolic NADP-isocitrate dehydrogenase antibody
  • ICDH antibody
  • IDCD antibody
  • IDH antibody
  • Idh1 antibody
  • IDHC_HUMAN antibody
  • IDP antibody
  • IDPC antibody
  • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
  • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
  • NADP dependent isocitrate dehydrogenase cytosolic antibody
  • NADP dependent isocitrate dehydrogenase peroxisomal antibody
  • NADP(+)-specific ICDH antibody
  • Oxalosuccinate decarboxylase antibody
  • PICD antibody
see all

Anti-Isocitrate dehydrogenase antibody images:

  Western blot - Isocitrate dehydrogenase antibody (ab81653)

Western blot - Isocitrate dehydrogenase antibody (ab81653)

Anti-Isocitrate dehydrogenase antibody (ab81653) at 1 µg/ml + 721_B cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 47 kDa
Observed band size : 47 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isocitrate dehydrogenase antibody (ab81653)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isocitrate dehydrogenase antibody (ab81653)

ab81653 at 5 µg/ml staining Isocitrate dehydrogenase in Human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isocitrate dehydrogenase antibody (ab81653)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isocitrate dehydrogenase antibody (ab81653)

ab81653 at 5 µg/ml staining Isocitrate dehydrogenase in Human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-Isocitrate dehydrogenase antibody (ab81653)

ab81653 has not yet been referenced specifically in any publications.

Publishing research using ab81653? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"