|
|
|
|
Western blot - Isocitrate dehydrogenase antibody (ab81653)

(enlarge)
|
|
|
|
|
Product Name
|
Isocitrate dehydrogenase antibody
|
| |
See all Isocitrate dehydrogenase antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Isocitrate dehydrogenase
|
|
Immunogen
|
Synthetic peptide from within residues: LGMMTSVLVC PDGKTVEAEA AHGTVTRHYR MYQKGQETST NPIASIFAWT, corresponding to internal sequence amino acids 288-337 of Human Isocitrate dehydrogenase (NP_005887)
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. ELISA titre using peptide based assay: 1/1562500.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
721_B cell lysate
|
|
Cellular localization
|
Cytoplasm. Peroxisome.
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins Signal Transduction >> Metabolism >> Energy Metabolism
|
|
Relevance
|
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: None Constituents: 2% Sucrose, PBS
|
|
Form
|
Lyophilised Add 50ul distilled water
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Isocitrate dehydrogenase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Isocitrate dehydrogenase antibody images, references, products related to ab81653 and other tools.
|
| |
Isocitrate dehydrogenase antibody images:
|
Western blot - Isocitrate dehydrogenase antibody (ab81653)
Isocitrate dehydrogenase antibody (ab81653) at 1 µg/ml + 721_B cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 47 kDa Observed band size : 47 kDa Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
|
| |
| |
|
Search PubMed (MEDLINE) for references to Isocitrate dehydrogenase
|
| |
Isocitrate dehydrogenase antibody - more information
|
Customer reviews (feedback) regarding Isocitrate dehydrogenase antibody |
Customer FAQs regarding Isocitrate dehydrogenase antibody |
Protocols for Isocitrate dehydrogenase antibody |
Price and availability of products related to Isocitrate dehydrogenase antibody |
| |
Isocitrate dehydrogenase antibody - related products:
|
|
|
| |
Isocitrate dehydrogenase antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Isocitrate dehydrogenase antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"