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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Isocitrate dehydrogenase antibody
See all Isocitrate dehydrogenase products (8) ...
Description
Rabbit polyclonal to Isocitrate dehydrogenase
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Cow, Cat, Dog, Pig, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide from within residues: LGMMTSVLVC PDGKTVEAEA AHGTVTRHYR MYQKGQETST NPIASIFAWT, corresponding to internal sequence amino acids 288-337 of Human Isocitrate dehydrogenase (NP_005887)
Positive control
721_B cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Energy Metabolism
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Western blot - Isocitrate dehydrogenase antibody (ab81653)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isocitrate dehydrogenase antibody (ab81653)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isocitrate dehydrogenase antibody (ab81653)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 47 kDa.
IHC-P: Use a concentration of 5 µg/ml
Target
Involvement in disease
Defects in IDH1 are involved in the development of glioma (GLM) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. Elevated levels of R(-)-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Cellular localization
Cytoplasm. Peroxisome.
Target information above from: UniProt accessionO75874
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- ICDH antibody
see all
Database links
- Entrez Gene: 281235 Cow
- Entrez Gene: 478889 Dog
- Entrez Gene: 3417 Human
- Entrez Gene: 15926 Mouse
- Entrez Gene: 24479 Rat
- Entrez Gene: 443257 Sheep
- Omim: 147700 Human
- SwissProt: Q9XSG3 Cow
see all
Anti-Isocitrate dehydrogenase antibody images:
Western blot - Isocitrate dehydrogenase antibody (ab81653)
Anti-Isocitrate dehydrogenase antibody (ab81653) at 1 µg/ml + 721_B cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 47 kDa
Observed band size : 47 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isocitrate dehydrogenase antibody (ab81653)
ab81653 at 5 µg/ml staining Isocitrate dehydrogenase in Human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Isocitrate dehydrogenase antibody (ab81653)
ab81653 at 5 µg/ml staining Isocitrate dehydrogenase in Human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References for Anti-Isocitrate dehydrogenase antibody (ab81653)
ab81653 has not yet been referenced specifically in any publications.
Publishing research using ab81653? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"