Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Isocitrate dehydrogenase antibody
See all Isocitrate dehydrogenase products (8) ...
Description
Rabbit polyclonal to Isocitrate dehydrogenase
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human, Pig
Predicted to work with
Mouse
Immunogen
Purified Isocitrate dehydrogenase from Pig heart.
Positive control
Heart tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Energy Metabolism
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Isocitrate dehydrogenase antibody (ab85532)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 47 kDa.
ELISA
ELISA: 1/400 - 1/1600.ELISA: 1/400 - 1/1600.
IHC-P: Use a concentration of 10 µg/ml
Target
Involvement in disease
Defects in IDH1 are involved in the development of glioma (GLM) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. Elevated levels of R(-)-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Cellular localization
Cytoplasm. Peroxisome.
Target information above from: UniProt accessionO75874
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- ICDH antibody
see all
Database links
- Entrez Gene: 3417 Human
- Entrez Gene: 15926 Mouse
- Omim: 147700 Human
- SwissProt: O75874 Human
- SwissProt: O88844 Mouse
- SwissProt: P20304 Pig
- Unigene: 593422 Human
- Unigene: 9925 Mouse
Anti-Isocitrate dehydrogenase antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Isocitrate dehydrogenase antibody (ab85532)
ab85532 at 10µg/ml staining Isocitrate dehydrogenase in human heart tissue, by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue
References for Anti-Isocitrate dehydrogenase antibody (ab85532)
ab85532 has not yet been referenced specifically in any publications.
Publishing research using ab85532? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"