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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Anti-JAB1 antibody [2A10] (ab495)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab495? Please let us know so that we can cite the reference in this datasheet
ab495 has been referenced in 2 publications.
- Cayli S et al. Developmental expression of p97/VCP (Valosin-containing protein) and Jab1/CSN5 in the rat testis and epididymis. Reprod Biol Endocrinol 9:117 (2011). IHC-P; Rat. PubMed: 21854589
- Calligé M et al. CSN5/Jab1 is involved in ligand-dependent degradation of estrogen receptor {alpha} by the proteasome. Mol Cell Biol 25:4349-58 (2005). PubMed: 15899841
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
](/ps/datasheet/images/0/ab495/JAB1-Primary-antibodies-ab495-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-JAB1 antibody [2A10](ab495)
IHC image of ab495 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab495, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![JAB1 antibody [2A10] for WB in Mouse (495)](/ps/datasheet/images/0/ab495/JAB1-Primary-antibodies-ab495-1.jpg)
JAB1 antibody [2A10] for WB in Mouse (495)
![JAB1 antibody [2A10] for ICC/IF in Mouse (495)](/ps/datasheet/images/0/ab495/JAB1-Primary-antibodies-ab495-3.jpg)
JAB1 antibody [2A10] for ICC/IF in Mouse (495)
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