 image](#JAB1-Primary-antibodies-ab495-4.jpg)
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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Overview
Product name
Anti-JAB1 antibody [2A10]
See all JAB1 products (12) ...
Description
Mouse monoclonal [2A10] to JAB1
Tested applications
WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Fusion protein encoding the full-length (amino acids 1-334) mouse JAB-1 gene expressed in E. coli.
Positive control
Recombinant JAB-1 protein, 293 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified from hybridoma cell culture supernatant by protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.
Clonality
Monoclonal
Clone number
2A10
Myeloma
NS1
Isotype
IgG2b
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Deubiquitination
Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> SCF Complex E3 Ligase
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-JAB1 antibody [2A10](ab495)
 image](/ps/datasheet/images/0/ab495/JAB1-Primary-antibodies-ab495-4.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.
IP: Use a concentration of 0.5 - 1 µg/ml.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex.
Sequence similarities
Belongs to the peptidase M67A family. CSN5 subfamily.
Contains 1 MPN (JAB/Mov34) domain.
Domain
The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ92905
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 38 kDa Mov34 homolog antibody
- COP9 (constitutive photomorphogenic) homolog subunit 5 antibody
- COP9 constitutive photomorphogenic homolog subunit 5 antibody
see all
Database links
- Entrez Gene: 10987 Human
- Entrez Gene: 26754 Mouse
- Entrez Gene: 312916 Rat
- Omim: 604850 Human
- SwissProt: Q92905 Human
- SwissProt: O35864 Mouse
- Unigene: 491912 Human
- Unigene: 402384 Mouse
see all
Anti-JAB1 antibody [2A10] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-JAB1 antibody [2A10](ab495)
IHC image of ab495 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab495, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-JAB1 antibody [2A10] (ab495)
This product has been referenced in:
- Cayli Set al. Developmental expression of p97/VCP (Valosin-containing protein) and Jab1/CSN5 in the rat testis and epididymis. Reprod Biol Endocrinol 9:117 (2011). IHC-P; Rat.Read more (PubMed: 21854589) »
- Calligé Met al. CSN5/Jab1 is involved in ligand-dependent degradation of estrogen receptor {alpha} by the proteasome. Mol Cell Biol 25:4349-58 (2005).Read more (PubMed: 15899841) »
See all 2 publications for this product
Publishing research using ab495? Please let us know so that we can cite the reference in this datasheet
![JAB1 antibody [2A10] for WB in Mouse (495)](/ps/datasheet/images/0/ab495/JAB1-Primary-antibodies-ab495-1.jpg)
![JAB1 antibody [2A10] for ICC/IF in Mouse (495)](/ps/datasheet/images/0/ab495/JAB1-Primary-antibodies-ab495-3.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"