JAB1 antibody (ab12185)

Product Name 

JAB1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to JAB1

Immunogen 

Synthetic peptide: SEYQTIPLNKIEDFGV conjugated to KLH, corresponding to amino acids 201-216 of Human JAB1. The corresponding sequence is identical in mouse and rat.
BLAST 'SEYQTIPLNKIEDFGV' with or

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Dm
(Due to sequence homology)

Tested applications 
(see key)

IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-P: 1/100, this is recommended using formalin-fixed, paraffin-embedded sections of human breast carcinoma.
IP: Use at an assay dependent dilution. 20-40 µg of the antibody immunoprecipitates JAB1 from 0.45 mg of RIPA extracts of rat PC12 cells.
WB: 1/500, this is recommended using a whole extract of human A431 cells and mouse NIH-3T3 cells, and a chemiluminescent detection reagent. Predicted molecular weight: 38 kDa. Additional bands may be detected when blotting with some extract preparations. Detection of the JAB1 band is specifically inhibited with the immunizing peptide.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole extract of human A431 cells, mouse NIH-3T3 cells (Western blot).
Rat PC12 cells (immunoprecipitation).
Human breast carcinoma (indirect immunoperoxidase staining).

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> SCF Complex E3 Ligase
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53

Relevance 

Jun activation domain-binding protein 1 (JAB1) also designated COP9 subunit 5 (COPS5) or SGN5 is a coactivator of AP1 transcription factor that also promotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. JAB1 interacts with c-Jun AP1 containing complexes, and enhances transactivation from AP1 dependent promoters. It also interacts with Jun D but not with Jun B or v-Jun. JAB1 is highly conserved in evolution and is widely expressed in mammalian tissues. It is localized both the nucleus and the cytoplasm. It interacts with the cytoplasmic domain of the alphaL/beta2 integrin LFA1. Following LFA1 engagement the nuclear pool of JAB1 increases and activation of an AP1 driven promoter is enhanced. Interaction of JAB1 with the nuclear progesterone receptor and the steroid receptor activator (SRC1) was reported.
JAB1 is a stability and activity regulator of Hypoxia inducible factor 1 (HIF1), a transcription factor that controls activation of several genes responsive to the cellular oxygen tension. The macrophage migration inhibitory factor (MIF) associates with JAB1 in the cytosol near the plasma membrane. Endogenous MIF inhibits JAB1 induced AP1 transcriptional activity. JAB1 is a subunit of the COP9 regulatory complex. COP9 cleaves the ubiquitin-like protein Nedd8 from the Cul1 subunit of SCF ubiquitin ligases. A metalloprotease motif in JAB1 plays a role in this isopeptidase activity. Breakdown of the cyclin dependent kinase inhibitor p27^Kip1 is promoted by JAB1. The latter expression in several cancers inversely correlates with p27^Kip1 and may reflect tumor aggressiveness. A possible involvement of JAB1 in atherosclerosis was also reported. Involvement of JAB1 in degradation of the suppressors p53 and smad4 was described recently.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Purification notes 

Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.

Form 

Liquid

Concentration 

9.300 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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