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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Overview
Product name
Anti-JAB1 antibody
See all JAB1 products (12) ...
Description
Rabbit polyclonal to JAB1
Tested applications
WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide: SEYQTIPLNKIEDFGV conjugated to KLH, corresponding to amino acids 201-216 of Human JAB1. The corresponding sequence is identical in mouse and rat.
SEYQTIPLNK IEDFGV
Positive control
Whole extract of human A431 cells, mouse NIH-3T3 cells (Western blot). Rat PC12 cells (immunoprecipitation). Human breast carcinoma (indirect immunoperoxidase staining).
General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Deubiquitination
Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> SCF Complex E3 Ligase
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IP
IHC-P
Application notes
IHC-P: 1/100, this is recommended using formalin-fixed, paraffin-embedded sections of human breast carcinoma.
IP: Use at an assay dependent dilution. 20-40 µg of the antibody immunoprecipitates JAB1 from 0.45 mg of RIPA extracts of rat PC12 cells.
WB: 1/500, this is recommended using a whole extract of human A431 cells and mouse NIH-3T3 cells, and a chemiluminescent detection reagent. Predicted molecular weight: 38 kDa. Additional bands may be detected when blotting with some extract preparations. Detection of the JAB1 band is specifically inhibited with the immunizing peptide.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex.
Sequence similarities
Belongs to the peptidase M67A family. CSN5 subfamily.
Contains 1 MPN (JAB/Mov34) domain.
Domain
The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ92905
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 38 kDa Mov34 homolog antibody
- COP9 (constitutive photomorphogenic) homolog subunit 5 antibody
- COP9 constitutive photomorphogenic homolog subunit 5 antibody
see all
Database links
- Entrez Gene: 42000 Fruit fly (Drosophila melanogaster)
- Entrez Gene: 10987 Human
- Entrez Gene: 26754 Mouse
- Entrez Gene: 312916 Rat
- Omim: 604850 Human
- SwissProt: Q9XZ58 Fruit fly (Drosophila melanogaster)
- SwissProt: Q92905 Human
- SwissProt: O35864 Mouse
see all
References for Anti-JAB1 antibody (ab12185)
ab12185 has not yet been referenced specifically in any publications.
Publishing research using ab12185? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"