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Western blot - JAB1 antibody (ab12323)
(enlarge)
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Product Name
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JAB1 antibody
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See all JAB1 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to JAB1
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Immunogen
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Immunogen was a synthetic peptide, which represented a c-terminal domain of human JAB1 (LocusLink ID 10987).
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/5000 - 1/25000. Detects a band of approximately 33, 36 kDa (predicted molecular weight: 40 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HEK 293T cells.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> SCF Complex E3 Ligase
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
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Relevance
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Jun activation domain-binding protein 1 (JAB1) also designated COP9 subunit 5 (COPS5) or SGN5 is a coactivator of AP1 transcription factor that also promotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. JAB1 interacts with c-Jun AP1 containing complexes, and enhances transactivation from AP1 dependent promoters. It also interacts with Jun D but not with Jun B or v-Jun. JAB1 is highly conserved in evolution and is widely expressed in mammalian tissues. It is localized both the nucleus and the cytoplasm. It interacts with the cytoplasmic domain of the alphaL/beta2 integrin LFA1. Following LFA1 engagement the nuclear pool of JAB1 increases and activation of an AP1 driven promoter is enhanced. Interaction of JAB1 with the nuclear progesterone receptor and the steroid receptor activator (SRC1) was reported.
JAB1 is a stability and activity regulator of Hypoxia inducible factor 1 (HIF1), a transcription factor that controls activation of several genes responsive to the cellular oxygen tension. The macrophage migration inhibitory factor (MIF) associates with JAB1 in the cytosol near the plasma membrane. Endogenous MIF inhibits JAB1 induced AP1 transcriptional activity. JAB1 is a subunit of the COP9 regulatory complex. COP9 cleaves the ubiquitin-like protein Nedd8 from the Cul1 subunit of SCF ubiquitin ligases. A metalloprotease motif in JAB1 plays a role in this isopeptidase activity. Breakdown of the cyclin dependent kinase inhibitor p27Kip1 is promoted by JAB1. The latter expression in several cancers inversely correlates with p27Kip1 and may reflect tumor aggressiveness. A possible involvement of JAB1 in atherosclerosis was also reported. Involvement of JAB1 in degradation of the suppressors p53 and smad4 was described recently.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Tris-citrate/phosphate. pH 7 to 8. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified using the peptide immobilized on solid support.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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Notes
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Centrifuge tube to remove product from lid.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JAB1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for JAB1 antibody images, references, products related to ab12323 and other tools.
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JAB1 antibody images:
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 Western blot - JAB1 antibody (ab12323)
Predicted band size : 40 kDa
Detection of Human CSN5 by Western Blot. Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T). Antibody: Affinity purified rabbit anti-CSN5 antibody BL1062 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
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References for JAB1 antibody (ab12323)
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This product has been used in:
Chew EH & Hagen T Substrate-mediated regulation of cullin neddylation. J Biol Chem 282:17032-40 (2007). WB; Human. PubMed: 17439941
If you publish research using ab12323 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to JAB1
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JAB1 antibody - more information
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