![Western blot - JAK1 antibody [AF28B1] (ab75744) image](#ab76841wb.jpg)
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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-JAK1 antibody [AF28B1]
See all JAK1 products (8) ...
Description
Mouse monoclonal [AF28B1] to JAK1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant full length protein (Human), purified from E.coli (His-JAK1).
Positive control
HeLa cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Clonality
Monoclonal
Clone number
AF28B1
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Other
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Western blot - JAK1 antibody [AF28B1] (ab75744)
![Western blot - JAK1 antibody [AF28B1] (ab75744) image](/ps/datasheet/Images/75/ab75744/ab76841wb.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab75744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Predicted molecular weight: 132 kDa.
ELISA: Use at an assay dependent dilution.
Target
Function
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Tissue specificity
Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Domain
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1.
Cellular localization
Endomembrane system. Wholly intracellular, possibly membrane associated.
Target information above from: UniProt accessionP23458
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- JAK 1 antibody
- JAK 1A antibody
- JAK 1B antibody
see all
Database links
- Entrez Gene: 3716 Human
- Entrez Gene: 16451 Mouse
- Entrez Gene: 84598 Rat
- Omim: 147795 Human
- SwissProt: P23458 Human
- SwissProt: P52332 Mouse
- SwissProt: Q5FVF5 Rat
- Unigene: 207538 Human
see all
Anti-JAK1 antibody [AF28B1] images:
Western blot - JAK1 antibody [AF28B1] (ab75744)
All lanes : Anti-JAK1 antibody [AF28B1] (ab75744) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : C6 cell lysate
Lane 4 : L929 cell lysate
Predicted band size : 132 kDa
References for Anti-JAK1 antibody [AF28B1] (ab75744)
This product has been referenced in:
- Kim TMet al. A developmental taxonomy of glioblastoma defined and maintained by MicroRNAs. Cancer Res 71:3387-99 (2011). WB; Human.Read more (PubMed: 21385897) »
See 1 publication for this product
Publishing research using ab75744? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"