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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab47435? Please let us know so that we can cite the reference in this datasheet
ab47435 has been referenced in 1 publications.
- Sutherland JM et al. Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial follicle activation. J Cell Physiol 227:1188-98 (2012). IHC-P; Mouse. PubMed: 21604262
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Paraffin-embedded sections) - JAK1 (phospho Y1022) antibody (ab47435)
Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab47435 at dilution of 1/50-1/100. Right panel treated with peptide and left panel untreated
Western blot - JAK1 antibody (ab47435)
All lanes : Anti-JAK1 antibody (ab47435) at 1/500 dilution
Lane 1 : MCF7 cell extract treated with EGF
Lane 2 : MCF7 cell extract treated with EGF with peptide
Predicted band size : 132 kDa
Immunocytochemistry/ Immunofluorescence-JAK1 antibody(ab47435)
ICC/IF image of ab47435 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47435, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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