Anti-JAK1 antibody (ab47435)

Dear Sir/Madam,   I purchased your antibody ab-47435 and despite following your protocols and recommended antibody concentrations it has failed to work on both western blot and immunofluorescence, two applications you claim this antibody works for. For this reason I would like to kindly request either a refund of the money paid or a substitute product (antibody) that will work to detect human Jak1 in western blot and immunofluorescence.   With best wishes,  

ANSWER:

Please accept my apologies for you having trouble with ab47435. The anti-JAK1 antibody is covered under our Abpromise, which states that we guarantee all of our antibodies for 6 months to work as stated on the datasheet. In the case of ab47435, it is guaranteed to work in ICC and WB on human samples. If the antibody fails to work as guaranteed if we cannot help resolve these issues, then will gladly replace the antibody or process a refund. I would be very happy to help you try and troubleshoot some of the issues you are having. To do this could you please provide me some information on the protocols that you have tried? For the western blot, are you seeing no bands when you develop your blot? Or are you seeing multiple bands, or high background? If you are seeing nothing, what primary antibody concentrations have you tried and what is the total protein concentration that you are loading on your gel? Also do you have a positive control on your gel? I ask as may be either increasing the antibody concentration or the protein concentration loaded onto the gel may help resolve seeing no bands. Incubating the antibody O/N at 4C would also be beneficial. If you are having issues with high background on your membrane, then if you are using milk as your blocking reagent, switching to BSA may help and vice-versa.

For the ICC, what tissue/cell samples are you using? Again depending on whether you are seeing no signal or high background, either increasing the antibody concentration or increasing the blocking (or changing the blocking reagent) could prove helpful.

As I mentioned previously, this antibody is covered under our Abpromise and so if we cannot resolve these issues I would be happy to send you another JAK1 antibody or a refund.

I look forward to your repl