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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-JAK1 antibody
See all JAK1 products (8) ...
Description
Rabbit polyclonal to JAK1
Specificity
Detects endogenous levels of JAK1 protein
Tested applications
ICC/IF, IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic non - phosphopeptide (Human) from around the phosphorylation site of Tyrosine 1022
Positive control
Human breast carcinoma tissue. Extracts from MCF7 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ or Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Other
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/10000ELISA: 1/10000
WB: 1/500 - 1/1000.Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
Target
Function
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Tissue specificity
Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Domain
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1.
Cellular localization
Endomembrane system. Wholly intracellular, possibly membrane associated.
Target information above from: UniProt accessionP23458
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 2.7.10.2 antibody
- JAK 1 antibody
- JAK 1A antibody
see all
Database links
- Entrez Gene: 3716 Human
- Entrez Gene: 16451 Mouse
- Entrez Gene: 84598 Rat
- Omim: 147795 Human
- SwissProt: P23458 Human
- SwissProt: P52332 Mouse
- SwissProt: Q5FVF5 Rat
- Unigene: 207538 Human
see all
Anti-JAK1 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - JAK1 (phospho Y1022) antibody (ab47435)
Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab47435 at dilution of 1/50-1/100. Right panel treated with peptide and left panel untreated
Western blot - JAK1 antibody (ab47435)
All lanes : Anti-JAK1 antibody (ab47435) at 1/500 dilution
Lane 1 : MCF7 cell extract treated with EGF
Lane 2 : MCF7 cell extract treated with EGF with peptide
Predicted band size : 132 kDa
Immunocytochemistry/ Immunofluorescence-JAK1 antibody(ab47435)
ICC/IF image of ab47435 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47435, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-JAK1 antibody (ab47435)
ab47435 has not yet been referenced specifically in any publications.
Publishing research using ab47435? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"