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Immunohistochemistry (Paraffin-embedded sections) - JAK1 (phospho Y1022) antibody (ab47435)
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Western blot - JAK1 antibody (ab47435)
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Immunocytochemistry/ Immunofluorescence-JAK1 antibody(ab47435)
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Product Name
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JAK1 antibody
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See all JAK1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to JAK1
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Immunogen
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Synthetic non - phosphopeptide (Human) from around the phosphorylation site of Tyrosine 1022
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Detects endogenous levels of JAK1 protein
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human breast carcinoma tissue.
Extracts from MCF7 cells.
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Cellular localization
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Intracytoplasmic membrane; peripheral membrane protein.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Other
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Relevance
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Janus kinase 1 (JAK1) is a member of a new class of non-receptor protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. It is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon recpeptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ or Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JAK1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for JAK1 antibody images, references, products related to ab47435 and other tools.
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JAK1 antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - JAK1 (phospho Y1022) antibody (ab47435)
Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab47435 at dilution of 1/50-1/100. Right panel treated with peptide and left panel untreated
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Western blot - JAK1 antibody (ab47435)
All lanes : JAK1 antibody (ab47435) at 1/500 dilution
Lane 1 : MCF7 cell extract treated with EGF
Lane 2 : MCF7 cell extract treated with EGF with peptide
Predicted band size : 132 kDa
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Immunocytochemistry/ Immunofluorescence-JAK1 antibody(ab47435)
ICC/IF image of ab47435 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47435, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to JAK1
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JAK1 antibody - more information
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Customer reviews (feedback) regarding JAK1 antibody |
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