Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-JAK1 antibody
See all JAK1 products (9) ...
Description
Rabbit polyclonal to JAK1
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Pig, Rhesus monkey
Immunogen
Synthetic peptide: KTLIEKERFYESRCRPVTPS-C, corresponding to amino acids 796-815 of Mouse JAK1
KTLIEKERFYESRCRPVTPS-C
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Other
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IP
Application notes
IP: Use at 5-10µl/1-2mg of lysate.
WB: 1/1000 - 1/2000. Predicted molecular weight: 132 kDa.
Note: The amount of JAK1 in cell lines tends to be extremely low. If the signal is weak it is recommended that a RIPA cell lysate (1-2mg protein) be prepared and JAK1 be first concentrated by immunoprecipitation and then immunoblotted. A degradation product of JAK1 (~110kDa) is also detected by the antiserum in immunoblots.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Tissue specificity
Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Domain
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1.
Cellular localization
Endomembrane system. Wholly intracellular, possibly membrane associated.
Target information above from: UniProt accessionP23458
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- JAK 1 antibody
- JAK 1A antibody
- JAK 1B antibody
see all
Database links
- Entrez Gene: 537201 Cow
- Entrez Gene: 3716 Human
- Entrez Gene: 16451 Mouse
- Entrez Gene: 397202 Pig
- Entrez Gene: 84598 Rat
- Omim: 147795 Human
- SwissProt: A3KMY9 Cow
- SwissProt: P23458 Human
see all
References for Anti-JAK1 antibody (ab79084)
ab79084 has not yet been referenced specifically in any publications.
Publishing research using ab79084? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"