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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-JAK1 (phospho Y1022) antibody
See all JAK1 products (8) ...
Description
Rabbit polyclonal to JAK1 (phospho Y1022)
Specificity
JAK1(Phospho-Tyr1022) Antibody detects endogenous levels of JAK1 only when phosphorylated at Tyrosine 1022.
Tested applications
IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Dog, Human
Immunogen
Synthetic peptide (Human) phosphopeptide derived from JAK1 around the phosphorylation site of Tyrosine 1022(K-E-YP-Y-T).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Other
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
Application notes
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Tissue specificity
Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Domain
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1.
Cellular localization
Endomembrane system. Wholly intracellular, possibly membrane associated.
Target information above from: UniProt accessionP23458
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 2.7.10.2 antibody
- JAK 1 antibody
- JAK 1A antibody
see all
Database links
- Entrez Gene: 442952 Dog
- Entrez Gene: 3716 Human
- Entrez Gene: 16451 Mouse
- Entrez Gene: 84598 Rat
- Omim: 147795 Human
- SwissProt: P23458 Human
- SwissProt: P52332 Mouse
- SwissProt: Q5FVF5 Rat
see all
Anti-JAK1 (phospho Y1022) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - JAK1 antibody (ab38519)
Analysis of breast carcinoma using ab 38519. Left: untreated. Right: treated with synthesised phosphopeptide
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - JAK1 (phospho Y1022) antibody (ab38519)
ab38519 staining JAK1 (phospho Y1022) in Dog lung tissue sections by IHC-P (Paraformaldehyde fixed paraffin embedded sections). Tissue was fixed with 10% buffered formalin and blocked with 5% serum for 20 minutes and 20°C. Antigen retrieval was by heat mediation in EDTA (pH8, 110°C, 2 minutes) buffer. Samples were incubated with primary antibody (1/200) for 1 hour at 20°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-JAK1 (phospho Y1022) antibody (ab38519)
ab38519 has not yet been referenced specifically in any publications.
Publishing research using ab38519? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"