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Anti-JAK1 (phospho Y1022) antibody (ab38519)

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Overview

Product name

Anti-JAK1 (phospho Y1022) antibody
See all JAK1 products (8) ...

Description

Rabbit polyclonal to JAK1 (phospho Y1022)

Specificity

JAK1(Phospho-Tyr1022) Antibody detects endogenous levels of JAK1 only when phosphorylated at Tyrosine 1022.

Tested applications

IHC-P, ELISAmore details

Cross reactivity

Reacts with

Mouse, Rat, Dog, Human

Immunogen

Synthetic peptide (Human) phosphopeptide derived from JAK1 around the phosphorylation site of Tyrosine 1022(K-E-YP-Y-T).

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Paraffin-embedded sections) - JAK1 antibody (ab38519)Immunohistochemistry (Paraffin-embedded sections) - JAK1 antibody (ab38519) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - JAK1 (phospho Y1022) antibody (ab38519)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - JAK1 (phospho Y1022) antibody (ab38519) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab38519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: 1/1000.
IHC-P: 1/50 - 1/100.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

Tissue specificity

Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.

Domain

Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1.

Cellular localization

Endomembrane system. Wholly intracellular, possibly membrane associated.

Target information above from: UniProt accessionP23458 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EC 2.7.10.2 antibody
  • JAK 1 antibody
  • JAK 1A antibody
  • JAK 1B antibody
  • JAK-1 antibody
  • JAK1 antibody
  • JAK1_HUMAN antibody
  • JAK1A antibody
  • JAK1B antibody
  • Janus kinase 1 (a protein tyrosine kinase) antibody
  • Janus Kinase 1 antibody
  • JTK3 antibody
  • Tyrosine protein kinase JAK 1 antibody
  • Tyrosine protein kinase JAK1 antibody
  • Tyrosine-protein kinase JAK1 antibody
see all

Anti-JAK1 (phospho Y1022) antibody images:

  Immunohistochemistry (Paraffin-embedded sections) - JAK1 antibody (ab38519)

Immunohistochemistry (Paraffin-embedded sections) - JAK1 antibody (ab38519)

Analysis of breast carcinoma using ab 38519. Left: untreated. Right: treated with synthesised phosphopeptide

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - JAK1 (phospho Y1022) antibody (ab38519)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - JAK1 (phospho Y1022) antibody (ab38519)

ab38519 staining JAK1 (phospho Y1022) in Dog lung tissue sections by IHC-P (Paraformaldehyde fixed paraffin embedded sections). Tissue was fixed with 10% buffered formalin and blocked with 5% serum for 20 minutes and 20°C. Antigen retrieval was by heat mediation in EDTA (pH8, 110°C, 2 minutes) buffer. Samples were incubated with primary antibody (1/200) for 1 hour at 20°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-JAK1 (phospho Y1022) antibody (ab38519)

ab38519 has not yet been referenced specifically in any publications.

Publishing research using ab38519? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"