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Immunohistochemistry (Paraffin-embedded sections) - JAK1 antibody (ab38519)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - JAK1 (phospho Y1022) antibody (ab38519)
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Product Name
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JAK1 (phospho Y1022) antibody
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See all JAK1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to JAK1 (phospho Y1022)
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Immunogen
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Synthetic peptide (Human) phosphopeptide derived from JAK1 around the phosphorylation site of Tyrosine 1022(K-E-YP-Y-T).
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Reacts with
(species key)
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Hu, Ms, Rat, Dog
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Specificity
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JAK1(Phospho-Tyr1022) Antibody detects endogenous levels of JAK1 only when
phosphorylated at Tyrosine 1022.
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Tested applications
(see key)
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ELISA, IHC-P, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Intracytoplasmic membrane; peripheral membrane protein.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Other
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Relevance
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Janus kinase 1 (JAK1) is a member of a new class of non-receptor protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. It is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon recpeptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JAK1 (phospho Y1022) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for JAK1 (phospho Y1022) antibody images, references, products related to ab38519 and other tools.
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JAK1 (phospho Y1022) antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - JAK1 antibody (ab38519)
Analysis of breast carcinoma using ab 38519. Left: untreated. Right: treated with synthesised phosphopeptide
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - JAK1 (phospho Y1022) antibody (ab38519)
ab38519 staining JAK1 (phospho Y1022) in Dog lung tissue sections by IHC-P (Paraformaldehyde fixed paraffin embedded sections). Tissue was fixed with 10% buffered formalin and blocked with 5% serum for 20 minutes and 20°C. Antigen retrieval was by heat mediation in EDTA (pH8, 110°C, 2 minutes) buffer. Samples were incubated with primary antibody (1/200) for 1 hour at 20°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody. This image is courtesy of an anonymous Abreview
See Abreview
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Search PubMed (MEDLINE) for references to JAK1
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JAK1 (phospho Y1022) antibody - more information
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JAK1 (phospho Y1022) antibody - related products:
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JAK1 (phospho Y1022) antibody - other tools:
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