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Anti-JAK2 antibody [691R5] (ab37226)

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1 question for ab37226

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Question 1

Monday 14-May-2012

To Whom It May Concern,
I am interested whether the mouse anti-JAK2 antibody (691R5,ab37226) can
be used for the detection of the following part of JAK2:

C-terminal end:
KYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNIL
VENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSK
SPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIR
DNMAG&

Thank you very much for your help!
Look forward to hearing from you!

Kind regards,

ANSWER:

  

Thank you for your inquiry.
The immunogen sequence for ab37226 is amino acid 745-955 of human JAK2.
The sequence you send me starts at amono acid 911.
So I can confirm that there is overlap of the two sequences. Unfortunately, ab37226 is a monoclonal antibody and the epitope it binds to has not been mapped any further.
Therefore I cannot guarantee that it will recognize the sequence you provided and therefore I cannot recommend it for your purposes.
I am sorry that I do not have a positive answer for your inquiry on this occasion and hope this information is nevertheless helpful.

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