JAK2 antibody [691R5] (ab37226)

Product Name 

JAK2 antibody [691R5]

Product type 

Primary antibodies

Description 

Mouse monoclonal [691R5] to JAK2

Immunogen 

Recombinant fragment from Human JAK2 expressed in E.coli.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 130 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human Jurkat cells, mouse 3T3L1 cells and rat L6 cells.

Cellular localization 

Intracellular, possibly membrane associated.

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other

Relevance 

JAK2 (Janus Activating Kinase 2) is a tyrosine kinase of the non-receptor type, that associates with the intracellular domains of cytokine receptors; JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin; it has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon.
Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1007 and 1008 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

691R5

Isotype 

IgG2b

Light chain type 

kappa

Purity 

Protein A purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from ascites by affinity chromatography.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for JAK2 antibody [691R5] (ab37226)

  This product has been used in: (two most recent references)

Clements D et al. Analysis of the oestrogen response in an angiomyolipoma derived xenograft model. Endocr Relat Cancer 16:59-72 (2009). PubMed: 18988705

Navarro A et al. Regulation of JAK2 by miR-135a: prognostic impact in classic Hodgkin lymphoma. Blood 114:2945-51 (2009). WB; Human. PubMed: 19666866
If you publish research using ab37226 please let us know so that we can cite the reference on this datasheet.

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