JAK2 antibody (ab39636)

Product Name 

JAK2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to JAK2

Immunogen 

Synthetic peptide (Human) derived from JAK2 around the phosphorylation site of Tyrosine 221.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab39636 detects endogenous levels of total JAK2 protein.

Tested applications 
(see key)

ELISA, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/20,000.
IHC-P: 1/50 - 1/100.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human breast carcinoma.

Cellular localization 

Intracellular, possibly membrane associated.

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other

Relevance 

JAK2 (Janus Activating Kinase 2) is a tyrosine kinase of the non-receptor type, that associates with the intracellular domains of cytokine receptors; JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin; it has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon.
Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1007 and 1008 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

Affinity purified using epitope-specific immunogen.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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