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Immunohistochemistry (Paraffin-embedded sections) - JAK2 antibody (ab47572)
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Product Name
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JAK2 antibody
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See all JAK2 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to JAK2
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Immunogen
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Synthetic non-phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 1007 (K-E-YP-Y-K).
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody detects endogenous levels of total JAK2 protein, and may cross react with phospho Jak1.
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Tested applications
(see key)
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ELISA, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10,000.
IHC-P: 1/50 - 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human breast carcinoma tissue.
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Cellular localization
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Intracellular, possibly membrane associated.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Relevance
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JAK2 (Janus Activating Kinase 2) is a tyrosine kinase of the non-receptor type, that associates with the intracellular domains of cytokine receptors; JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin; it has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon.
Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1007 and 1008 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JAK2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for JAK2 antibody images, references, products related to ab47572 and other tools.
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JAK2 antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - JAK2 antibody (ab47572)
This image shows human breast carcinoma stained with ab47572 at 1/50 dilution. Right hand image - sample was treated with immunogenic peptide. Left hand image - tissue without immunogenic peptide.
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Search PubMed (MEDLINE) for references to JAK2
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JAK2 antibody - more information
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