Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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ab32101 has been referenced in 6 publications.
Publishing research using ab32101? Please let us know so that we can cite the reference in this datasheet
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Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132] (ab32101) at 1/50 dilution + immunoprecipitation pellets from Jurkat cell lysate
Predicted band size : 130 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
ab32101 staining JAK-2 on human lung adenocarcinoma in IHC-P.
Analysis of immunogen phosphopeptide (A) and non phosphopeptide (B). Antibody used at 1/1000 dilution.
All lanes : Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132] (ab32101) at 1/2000 dilution
Lane 1 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 0 hours.
Lane 2 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 15 minutes.
Lane 3 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 30 minutes.
Lane 4 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 1 hour.
Lane 5 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 2 hours.
Lane 6 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 4 hours.
Lane 7 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 6 hours.
Lane 8 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 24 hours.
Lysates/proteins at 30 µg per lane.
Secondary
An HRP-conjugated donkey anti-rabbit polyclonal. at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 130 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa (possible non-specific binding).
This image is courtesy of an anonymous Abreview
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