JAK2 (phospho Y1007 + Y1008) antibody [E132] (ab32101)

Product Name 

JAK2 (phospho Y1007 + Y1008) antibody [E132]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [E132] to JAK2 (phospho Y1007 + Y1008)

Immunogen 

Synthetic phosphopeptide corresponding to residues surrounding Tyr 1007 and Tyr 1008 of human JAK2

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Only detects JAK2 phosphorylated on Tyrosine 1007 and 1008

Tested applications 
(see key)

Dot, ICC, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Dot: 1/1000.

ICC: 1/50 - 1/100.
IHC-P: 1/50 - 1/100.
IP: 1/50.
WB: 1/5000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 130 kDa).

Is unsuitable for FACS.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Intracellular, possibly membrane associated.

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other

Relevance 

JAK2 (Janus Activating Kinase 2) is a tyrosine kinase of the non-receptor type, that associates with the intracellular domains of cytokine receptors; JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin; it has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon.
Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1007 and 1008 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

E132

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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References for JAK2 (phospho Y1007 + Y1008) antibody [E132] (ab32101)

  This product has been used in: (two most recent references)

Dawson MA et al. JAK2 phosphorylates histone H3Y41 and excludes HP1alpha from chromatin. Nature 461:819-22 (2009). WB; Human. PubMed: 19783980

Devi YS et al. Regulation of transcription factors and repression of Sp1 by prolactin signaling through the short isoform of its cognate receptor. Endocrinology 150:3327-35 (2009). PubMed: 19342455
If you publish research using ab32101 please let us know so that we can cite the reference on this datasheet.

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