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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Overview
Product name
Anti-JAKMIP2 antibody
Description
Rabbit polyclonal to JAKMIP2
Specificity
ab65019 detects endogenous levels of total JAKMIP2 protein.
Tested applications
ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from the C-terminal of human JAKMIP2.
Positive control
Human brain tissue. LOVO and A549 cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab65019 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000.Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Target
Tissue specificity
Highly expressed in brain, moderately expressed in thymus, spleen and lung, and weakly expressed in kidney, liver and peripheral blood lymphocytes. Aslo expressed in adrenal and pituitary glands, as well as testis.
Sequence similarities
Belongs to the JAKMIP family.
Cellular localization
Golgi apparatus.
Target information above from: UniProt accessionQ96AA8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CTCL tumor antigen HD CL 04 antibody
- CTCL tumor antigen HD-CL-04 antibody
- Jak and microtubule interacting protein 2 antibody
see all
Database links
- Entrez Gene: 9832 Human
- Omim: 611197 Human
- SwissProt: Q96AA8 Human
- Unigene: 184323 Human
Anti-JAKMIP2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - JAKMIP2 antibody (ab65019)
ab65019, at 1/50 - 1/100 dilution, staining JAKMIP2 in human brain by immunohistochemistry using paraffin-embedded tissue, in the absence (left panel) or presence (right panel) of the immunising peptide.
Western blot - JAKMIP2 antibody (ab65019)
All lanes : Anti-JAKMIP2 antibody (ab65019) at 1/500 dilution
Lane 1 : LOVO cell extract
Lane 2 : A549 cell extract
Lane 3 : A549 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 95 kDa
Observed band size : 95 kDa
References for Anti-JAKMIP2 antibody (ab65019)
ab65019 has not yet been referenced specifically in any publications.
Publishing research using ab65019? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"