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Western blot - JAML antibody (ab67843)
(enlarge)
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Product Name
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JAML antibody
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to JAML
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Immunogen
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JAML (AAI00798.1, 1 a.a. ~ 355 a.a) full-length human protein.
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Reacts with
(species key)
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Localized at the plasma membrane in areas of cell-cell contacts and at the cell surface of PMN
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Immunology >> Cell Type Markers >> CD >> Adhesion
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial
Immunology >> Cell Type Markers >> CD >> Myeloid Cells
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Relevance
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JAML (junctional adhesion molecule-like) mediates adhesive interactions with CXADR, a protein of the junctional complex of epithelial cells, via the Ig-like V-type domain. It also enhances myeloid leukemia cell adhesion to endothelial cells. It is expressed at the plasma membrane of polymorphonuclear leukocytes and expression is restricted to the hematopoietic tissues with the exception of liver. It is also expressed in fetal liver, spleen and thymus. JAML is up-regulated upon retinoic acid, Me2SO and PMA treatment in differentiating myeloid leukemia cells. The gene name for JAML is AMICA (adhesion molecule that interacts with CXADR antigen 1).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.2
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JAML antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for JAML antibody images, references, products related to ab67843 and other tools.
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JAML antibody images:
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 Western blot - JAML antibody (ab67843)
All lanes : JAML antibody (ab67843) at 1/500 dilution
Lane 1 : JAML transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
antibody at 1/2500 dilution
developed using the ECL technique
Predicted band size : 44 kDa
Observed band size : 44 kDa
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Search PubMed (MEDLINE) for references to JAML
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JAML antibody - more information
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Customer reviews (feedback) regarding JAML antibody |
Customer FAQs regarding JAML antibody |
Protocols for JAML antibody |
Price and availability of products related to JAML antibody |
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JAML antibody - related products:
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JAML antibody - other tools:
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Contact Abcam with a Technical Enquiry about JAML antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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