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Western blot - JAZF1 antibody (ab80329)
Western blot - JAZF1 antibody (ab80329)
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Product Name 

JAZF1 antibody

 

See all JAZF1 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to JAZF1

Immunogen 

Synthetic peptide: CKKRYKNVNG IKYHAKNGHR TQIRVRKPFK CRCGKSYKTA QGLRHHTINF , corresponding to C terminal amino acids 181-230 of Human JAZF1 (accession number: NP_778231)
BLAST 'CKKRYKNVNG IKYHAKNGHR TQIRVRKPFK CRCGKSYKTA QGLRHHTINF ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu, Ms, Rat

Predicted to react 
(species key)

Zfsh, Orngt
(Due to sequence homology)

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1:312500

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat nuclear lysate

Cellular localization 

Nucleus

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

JAZF1 is a nuclear protein with three C2H2-type zinc fingers. It functions as a transcriptional repressor. Chromosomal aberrations involving this gene are associated with endometrial stromal tumors. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

221895    (Human)
231986    (Mouse)
685879    (Rat)

Omim

    

606246    (Human)

SwissProt

    

Q86VZ6    (Human)
Q80ZQ5    (Mouse)
Q5RDF5    (Orangutan)

Unigene

    

368944    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Lyophilised
Add 50 µl of distilled water for a final antibody concentration of 1 mg/ml.

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JAZF1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for JAZF1 antibody images, references, products related to ab80329 and other tools.

 

JAZF1 antibody images:

Product image  Western blot - JAZF1 antibody (ab80329)

Western blot - JAZF1 antibody (ab80329)

JAZF1 antibody (ab80329) at 1 µg/ml + Jurkat cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 27 kDa

 
 

Search PubMed (MEDLINE) for references to JAZF1

 

JAZF1 antibody - more information

Customer reviews (feedback) regarding JAZF1 antibody

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Protocols for JAZF1 antibody

Price and availability of products related to JAZF1 antibody

 

JAZF1 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for JAZF1 antibody

ab14844

  

Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate

 

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Other JAZF1 Antibodies

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JAZF1 antibody - other tools:

Contact Abcam with a Technical Enquiry about JAZF1 antibody

 

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