JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) (ab112134)
- Product nameJC-10 Mitochondrial Membrane Potential Assay Kit (Microplate)See all Mitochondrial Transmembrane Potential kits ...
- Tests5 x 96 well plate
- Sample typeAdherent cells, Suspension cells
- Assay typeDirect
- Product overview
Although JC-1 is widely used in many labs, its poor water solubility causes great inconvenience. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 is developed to be a superior alternative to JC-1 when high dye concentration is desired. Compared to JC-1, JC-10 has much better water solubility. JC-10 is capable of selectively entering into mitochondria, and reversibly changes its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e., emission of JC-10 monomeric form) to 570 nm (i.e., emission of J-aggregate form). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized. Both colors can be detected using the filters commonly mounted in all flow cytometers. The green emission can be analyzed in fluorescence channel 1 (FL1) and greenish orange emission in channel 2 (FL2). Besides its use in flow cytometry, it can also be used in fluorescence imaging and fluorescence microplate platform.
ab112134 Mitochondria Membrane Potential Assay Kit enables researchers to run JC-10 assay in the format of microplate reader, and it provides the most robust assay method for monitoring mitochondria membrane potential changes.
ab112134 is based on the detection of the mitochondrial membrane potential changes in cells by the cationic, lipophilic JC-10 dye. In normal cells, JC-10 concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. However, in apoptotic and necrotic cells, JC-10 diffuses out of mitochondria. It changes to monomeric form and stains cells in green fluorescence.
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- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 5 x 96 tests 100X JC-10 in DMSO 1 x 250µl Assay Buffer A 1 x 25ml Assay Buffer B 1 x 25ml
- RelevanceMitochondrial Transmembrane Potential is an important parameter of mitochondrial function used as an indicator of cell death. The collapse of the mitochondrial transmembrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
- mitochondrial membrane potential
Our Abpromise guarantee covers the use of ab112134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) images
Camptothecin-induced mitochondria membrane potential changes with JC-10 and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 µM) for 4 hours, JC-1 and JC-10 dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10 were measured at Ex/Em = 490/525 nm and 490/590 nm with a microplate reader.
References for JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) (ab112134)
ab112134 has not yet been referenced specifically in any publications.