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Product name
JC1 - Mitochondrial Membrane Potential Assay Kit
See all Mitochondrial Transmembrane Potential products (8) ...
Tests
1 x 96 test
Product overview
ab113850 uses tetraethylbenzimidazolylcarbocyanine iodide (JC-1), a cationic dye that accumulates in energized mitochondria. At low concentrations (due to low mitochondrial membrane potential) JC-1 is predominantly a monomer that yields green fluorescence with emission of 530±15nm. At high concentrations (due to high mitochondrial membrane potential) the dye aggregates yielding a red to orange colored emission (590±17.5nm). Therefore a decrease in the aggregate fluorescent count is indicative of depolarization whereas an increase is indicative of hyperpolarization.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at -20°C.
| Components | Identifier | 100 tests |
|---|---|---|
| Dilution Buffer (10X, sterile) | 1 x 10ml | |
| DMSO (cell culture tested) | P/N 8206008 | 1 x 1ml |
| FCCP (50mM) | 1 x 10µl | |
| JC-1 (lyophilized) | P/N 8206007 | 1 x 500µg |
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Kits/ Lysates/ Other >> Kits >> Cell Damage Kits >> Cell viability, plasma membrane damage
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Cell Viability and Senescence Kits
Kits/ Lysates/ Other >> Kits >> Apoptosis Kits >> Other Apoptosis Kits
Kits/ Lysates/ Other >> Kits >> Cell Damage Kits
Cell Biology >> Apoptosis >> Mitochondrial
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Functional Studies - JC1 - Mitochondrial Membrane Potential Assay Kit (ab113850)
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Relevance
Mitochondrial Transmembrane Potential (? Ψ) is an important parameter of mitochondrial function used as an indicator of cell death. The collpase of the (??) mitochondrial transmembrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
Alternative names
- MS959
Applications
Show applications key
Our Abpromise guarantee covers the use of ab113850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS
JC1 - Mitochondrial Membrane Potential Assay Kit images:
Functional Studies - JC1 - Mitochondrial Membrane Potential Assay Kit (ab113850)
ab113850 (JC1) labeled HL60 cells were seeded at 250000 cells per well in glucose based complete media and treated for 4 hours with 100µM FCCP and vehicle control (DMSO). Cells were then transferred to a microplate and read on a spectrophotometer. Mean and standard deviation is plotted for 3 replciates from each condition.
Protocols
References for JC1 - Mitochondrial Membrane Potential Assay Kit (ab113850)
ab113850 has not yet been referenced specifically in any publications.
Publishing research using ab113850? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"