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JCV Polyomavirus Major Capsid VP1 protein (ab74569)

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Overview

  • Product nameJCV Polyomavirus Major Capsid VP1 protein
  • Protein descriptionRecombinant full length JCV Polyomavirus Major Capsid VP1 protein expressed in S. cerevisiae, 44kDa. Swiss ID = P03089.
  • Expression hostSaccharomyces cerevisiae

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesPurified by ultracentifugation.
  • FormLyophilised:Reconstitute with deionized H2O. After reconstitution store at 4°C.
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab74569 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA
    WB
    SDS-PAGE
  • Application notesELISA: Use at a concentration of 1 - 5 µg/ml to coat a microtitre plate.
    WB: Use at a concentration of 1 µg/lane. Predicted molecular weight: 40 kDa.
    SDS-PAGE: Use at a concentration of 4 µg/lane.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Capsid protein VP1
  • RelevanceThe human polyomavirus JC virus (JCV) infects greater than 80% of the human population. The JC virus is a small (38-40 nm in diameter) double stranded, circular DNA virus covered by an icosahedral capsid. Infection with JCV is asymptomatic and it occurs in early childhood. After the primary infection, the virus remains in latent state in the kidney, until it's reactivation under immunosuppressive conditions to result in Progressive Multifocal Leukoencephalopathy (PML), a fatal demyelinating disease. 70% of all HIV-1- infected patients will exhibit neurological disorders and between 5 and 8% of all HIV-1-infected patients will develop PML. Similar to other polyomaviruses, JCV can cause tumors when intracerebrally inoculated at high titers into developing rodent. Several reports suggest the association of viruses, especially of the polyomavirus family with different types of human brain tumors. Tumorigenecity of JCV is most likely induced by the viral early gene product T-antigen. T-antigen has the capacity to interact with several tumor suppressor proteins, most notably p53, and functionally inactivate these proteins.
  • Cellular localizationVirion. Nucleus

    • JCV Polyomavirus Major Capsid VP1 protein images

    • SDS-PAGE - JCV Polyomavirus Major Capsid VP1 protein (ab74569)
      SDS-PAGE - JCV Polyomavirus Major Capsid VP1 protein (ab74569)
      SDS-PAGE showing ab74569 (4µg/lane).
      Lane 1 reprents the molecular weight ladder. From the bottom: 14.4, 18.4, 25.0, 35.0, 45.0, 66.2 kDa

    References for JCV Polyomavirus Major Capsid VP1 protein (ab74569)

    ab74569 has not yet been referenced specifically in any publications.

    Publishing research using ab74569 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab74569 Submit a Question for ab74569

    Displaying 1 - 3 of 3 results for Abreviews and Q&A

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    Wir wollen eine ELISA für humane Polyoma IgG's und IgM's aufbauen. Da ich kein Kit finde bin ich auf Ihre Polyoma Proteine und Antikörper aufmerksam geworden.

    Habe ich es richtig verstanden, dass das folgende Protein
    JCV Pol...

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    Die Spezifitätsnotiz beschreibt folgendes:

    ab34756 bindet das Major Capsidprotein VP1 von humanem Polyoma Virus JCV und das Capsidprotein VP1 von hamster polyomavirus (HaPyV) and Simian Virus 40 (SV40).

    ab34756 kreuz-rea...

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    Can I also detect ab74565 (SV40 VP1) with this mAb ab34756 (anti-Hu JCV)? Please confirm it.

    Thank you for your reply. I can confirm that the antibody ab34756 will react with the SV40 VP1 protein ab74565. On the antibody datasheet in the section labelled "cross-reactivity", it notes that it does react with the VP1 protein of SV40. I hope this wi...

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    Are these proteins tagged?

    Thank you for your call this week and for your patience while I have been in touch with the production lab about these proteins. I have confirmed with the lab that these proteins do not contain any tag. To detect them, you could use specific monoclonal an...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"