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JEG-3 (Human placental choriocarcinoma cell line) Nuclear Lysate (ab14841)

Q&A (1)

Overview

  • Product nameJEG-3 (Human placental choriocarcinoma cell line) Nuclear Lysate
  • General notesWe recommend aliquoting the extracts into single use fractions and then storing them.
  • Tested applicationsEMSA, WB more details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research Areas
  • BackgroundThe human placenta is a highly invasive tumor-like structure in which a subpopulation of placental trophoblast cells known as the "extravillous trophoblast" (EVT) invades the uterine decidua and its vasculature to establish adequate fetal-maternal exchange of molecules. In vitro-propagated short-lived EVT cell lines such as JEG-3 cells are used to study molecular mechanisms responsible for this invasiveness, which are identical to those of cancer cells; however, unlike cancer cells, their proliferation, migration, and invasiveness in situ are stringently controlled by decidua-derived transforming growth factor (TGF)-beta. These epithelial-trophoblast-like cells are therefore used in identifying genetic changes underlying epithelial tumor progression as well as gonadotrophin secretion studies.

    • Applications

    Our Abpromise guarantee covers the use of ab14841 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    EMSA
    WB
  • Application notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for JEG-3 (Human placental choriocarcinoma cell line) Nuclear Lysate (ab14841)

    ab14841 has not yet been referenced specifically in any publications.

    Publishing research using ab14841 ? Please let us know so that we can cite the reference in this datasheet

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    For the product ab14841 (JEG-3 cell line), do you know if it is HLA-G positive?

    Thank you for your enquiry. JEG-3 cells are known to express the HLA-G protein. Please note though that ab14841 is a nuclear lysate, so HLA-G protein levels are expected to be low, if at all. There are a lot of publications on JEG-3 expressing HLA...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"