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Overview
Product name
Anti-JHDM1D antibody
See all JHDM1D products (3) ...
Description
Rabbit polyclonal to JHDM1D
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 750 - 850 of Human JHDM1D.
(Peptide available as ab36043.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: Hela, Jurkat, HEK293.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - JHDM1D antibody (ab36044)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
Target
Function
Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate.
Sequence similarities
Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
Contains 1 JmjC domain.
Contains 1 PHD-type zinc finger.
Domain
The PHD-type zinc finger mediates the binding to H3K4me3. Binding to H3K4me3 prevents its access to H3K9me2.
The linker region is a critical determinant of demethylase specificity. It prevents the active site of JmjC to reach the target H3K9me2 when the PHD-type zinc finger binds to H3K4me3, while it favors selectivity toward H3K27me2.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ6ZMT4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- JHDM1D antibody
- jmjc containing protein antibody
- JmjC domain-containing histone demethylation protein 1D antibody
see all
Database links
- Entrez Gene: 80853 Human
- SwissProt: Q6ZMT4 Human
- Unigene: 308710 Human
Anti-JHDM1D antibody images:
Western blot - JHDM1D antibody (ab36044)
All lanes : Anti-JHDM1D antibody (ab36044) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 107 kDa
Observed band size : 107 kDa
Exposure time : 2 minutes
References for Anti-JHDM1D antibody (ab36044)
This product has been referenced in:
- Huang Cet al. Dual-specificity histone demethylase KIAA1718 (KDM7A) regulates neural differentiation through FGF4. Cell Res 20:154-65 (2010). WB; Human.Read more (PubMed: 20084082) »
See 1 publication for this product
Publishing research using ab36044? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"