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Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)
Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)
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Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)
Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)
(enlarge)


Western blot - JHDM1D antibody (ab37905)
Western blot - JHDM1D antibody (ab37905)
(enlarge)


Product Name 

JHDM1D antibody

 

See all JHDM1D antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to JHDM1D

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human JHDM1D.

(Peptide available as ab37904.)

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
ICC/IF: Use at a concentration of 5ug/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in the following Human whole cell lysates:
HeLa, Jurkat, HEK293, HepG2, MCF-7 and A431.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins

Relevance 

JHDM1D (KIAA1718) is a novel 107 kDa jumonji C containing protein that may play a unique role in histone demethylation.

 

Alternative names JHDM1D antibody (ab37905)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

80853    (Human)

SwissProt

    

Q6ZMT4    (Human)

Unigene

    

308710    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.400 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JHDM1D antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for JHDM1D antibody images, references, products related to ab37905 and other tools.

 

JHDM1D antibody images:

Product image  Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)

Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)

ICC/IF image of ab37905 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab37905, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).  However, this Fast-Track antibody is not yet fully characterised.  This image represents inconclusive preliminary data.

 

Product image  Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)

Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)

ICC/IF image of ab37905 stained MCF-7 cells. The cells were fixed with 100% Methanol (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37905 at 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

This Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data

 

Product image  Western blot - JHDM1D antibody (ab37905)

Western blot - JHDM1D antibody (ab37905)

All lanes : JHDM1D antibody (ab37905) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 107 kDa
Observed band size : 107 kDa
Additional bands at : 53 kDa,75 kDa. We are unsure as to the identity of these extra bands.

 
 

Search PubMed (MEDLINE) for references to JHDM1D

 

JHDM1D antibody - more information

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JHDM1D antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for JHDM1D antibody

ab37904

  

JHDM1D peptide

 

Compatible Secondaries for JHDM1D antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other JHDM1D Antibodies

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JHDM1D antibody - other tools:

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