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Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)
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Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)
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Western blot - JHDM1D antibody (ab37905)
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Product Name
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JHDM1D antibody
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See all JHDM1D antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to JHDM1D
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human JHDM1D. (Peptide available as ab37904.)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
ICC/IF: Use at a concentration of 5ug/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following Human whole cell lysates:
HeLa, Jurkat, HEK293, HepG2, MCF-7 and A431.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
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Relevance
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JHDM1D (KIAA1718) is a novel 107 kDa jumonji C containing protein that may play a unique role in histone demethylation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.400 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JHDM1D antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for JHDM1D antibody images, references, products related to ab37905 and other tools.
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JHDM1D antibody images:
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 Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)
ICC/IF image of ab37905 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab37905, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
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Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)
ICC/IF image of ab37905 stained MCF-7 cells. The cells were fixed with 100% Methanol (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37905 at 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
This Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data
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Western blot - JHDM1D antibody (ab37905)
All lanes : JHDM1D antibody (ab37905) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 107 kDa
Observed band size : 107 kDa
Additional bands at : 53 kDa,75 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to JHDM1D
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JHDM1D antibody - more information
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Customer reviews (feedback) regarding JHDM1D antibody |
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JHDM1D antibody - related products:
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JHDM1D antibody - other tools:
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Contact Abcam with a Technical Enquiry about JHDM1D antibody |
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