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Products:Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Overview
Product name
Anti-JHDM1D antibody
See all JHDM1D products (3) ...
Description
Rabbit polyclonal to JHDM1D
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human JHDM1D.
(Peptide available as ab37904.)
Positive control
This antibody gave a positive signal in the following Human whole cell lysates: HeLa, Jurkat, HEK293, HepG2, MCF-7 and A431.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
ICC/IF: Use at a concentration of 5ug/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate.
Sequence similarities
Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
Contains 1 JmjC domain.
Contains 1 PHD-type zinc finger.
Domain
The PHD-type zinc finger mediates the binding to H3K4me3. Binding to H3K4me3 prevents its access to H3K9me2.
The linker region is a critical determinant of demethylase specificity. It prevents the active site of JmjC to reach the target H3K9me2 when the PHD-type zinc finger binds to H3K4me3, while it favors selectivity toward H3K27me2.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ6ZMT4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- JHDM1D antibody
- jmjc containing protein antibody
- JmjC domain-containing histone demethylation protein 1D antibody
see all
Database links
- Entrez Gene: 80853 Human
- SwissProt: Q6ZMT4 Human
- Unigene: 308710 Human
Anti-JHDM1D antibody images:
Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)
ICC/IF image of ab37905 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab37905, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
Immunocytochemistry/ Immunofluorescence - JHDM1D antibody (ab37905)
ICC/IF image of ab37905 stained MCF-7 cells. The cells were fixed with 100% Methanol (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37905 at 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
This Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data
Western blot - JHDM1D antibody (ab37905)
All lanes : Anti-JHDM1D antibody (ab37905) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 107 kDa
Observed band size : 107 kDa
Additional bands at : 53 kDa,75 kDa. We are unsure as to the identity of these extra bands.
References for Anti-JHDM1D antibody (ab37905)
ab37905 has not yet been referenced specifically in any publications.
Publishing research using ab37905? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"