Anti-JLP antibody (ab12331)

Western blot - JLP antibody (ab12331)

Samples: Nuclear extract (NE, 20 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-JLP antibody ab12331 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Samples: Nuclear extract (NE, 20 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-JLP antibody ab12331 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunocytochemistry/ Immunofluorescence - JLP antibody (ab12331)

ICC/IF image of ab12331 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12331, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.