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Overview
Product name
Anti-JMJD1C antibody
See all JMJD1C products (4) ...
Description
Rabbit polyclonal to JMJD1C
Tested applications
IP, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1000 - 1100 of Human JMJD1C.
(Peptide available as ab32470.)
Positive control
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - JMJD1C antibody (ab31215)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab31215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution. (PubMed: 20530532)
IHC-P: Use at an assay dependent dilution. (PubMed: 20530532)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 285 kDa (predicted molecular weight: 285 kDa).
Target
Function
Probable histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May be involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes.
Sequence similarities
Belongs to the JHDM2 histone demethylase family.
Contains 1 JmjC domain.
Domain
Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ15652
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- JHD2C_HUMAN antibody
- Jmjd1c antibody
- jumonji domain containing 1C antibody
see all
Database links
- Entrez Gene: 221037 Human
- Entrez Gene: 108829 Mouse
- Omim: 604503 Human
- SwissProt: Q15652 Human
- SwissProt: Q69ZK6 Mouse
- SwissProt: Q6NV48 Mouse
- Unigene: 413416 Human
- Unigene: 499821 Human
see all
Anti-JMJD1C antibody images:
Western blot - JMJD1C antibody (ab31215)
Anti-JMJD1C antibody (ab31215) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 285 kDa
Observed band size : 285 kDa
References for Anti-JMJD1C antibody (ab31215)
This product has been referenced in:
- Kim SMet al. Regulation of mouse steroidogenesis by WHISTLE and JMJD1C through histone methylation balance. Nucleic Acids Res 38:6389-403 (2010). WB, IHC-P, IP; Mouse.Read more (PubMed: 20530532) »
See 1 publication for this product
Publishing research using ab31215? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"