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Western blot - JMJD4 antibody (ab84937)
Western blot - JMJD4 antibody (ab84937)
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Product Name 

JMJD4 antibody

 

See all JMJD4 antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to JMJD4

Immunogen 

Synthetic peptide within residues: APGRVAFVSE PGAFSYADFV RGFLLPNLPC VFSSAFTQGW GSRRRWVTPA , corresponding to N terminal amino acids 72-122 of Human JMJD4 (NP_075383)
BLAST 'APGRVAFVSE PGAFSYADFV RGFLLPNLPC VFSSAFTQGW GSRRRWVTPA ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human fetal heart lysate.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins

Relevance 

JMJD4 is a novel putative histone demethylase that contains a jumonji domain.

 

Alternative names JMJD4 antibody (ab84937)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

65094    (Human)

SwissProt

    

Q9H9V9    (Human)

Unigene

    

555974    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Purification notes 

ab84937 is purified by a peptide affinity chromatography method.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JMJD4 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for JMJD4 antibody images, references, products related to ab84937 and other tools.

 

JMJD4 antibody images:

Product image  Western blot - JMJD4 antibody (ab84937)

Western blot - JMJD4 antibody (ab84937)

JMJD4 antibody (ab84937) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

Secondary
anti-Rabbit IgG HRP at 1/50000 dilution

Predicted band size : 52 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)


Gel concentration 12%

 
 

Search PubMed (MEDLINE) for references to JMJD4

 

JMJD4 antibody - more information

Customer reviews (feedback) regarding JMJD4 antibody

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Protocols for JMJD4 antibody

Price and availability of products related to JMJD4 antibody

 

JMJD4 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for JMJD4 antibody

ab29428

  

Heart (Human) Nuclear Lysate - fetal normal tissue

 

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ab6564

  

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Chromeo™ 488 antibody

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Other JMJD4 Antibodies

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JMJD4 antibody - other tools:

Contact Abcam with a Technical Enquiry about JMJD4 antibody

 

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