Overview

  • Product name
  • Description
    Rabbit polyclonal to JMJD5
  • Specificity
    ab106391 is specific for JMJD5. At least two isoforms of JMJD5 are known to exist; this antibody should detect both.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human JMJD5 (C terminal). An 18 amino acid peptide from near the carboxy terminus of Human JMJD5.
    Database link: Q8N371

  • Positive control
    • WB: Human liver tissue lysate IHC-P: Human liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab106391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 47 kDa.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Function
    Histone demethylase required for G2/M phase cell cycle progression. Specifically demethylates dimethylated 'Lys-36' (H3K36me2) of histone H3, an epigenetic repressive mark, thereby acting as a transcription activator. Regulates expression of CCNA1 (cyclin-A1), leading to regulate cancer cell proliferation.
  • Tissue specificity
    Weakly expressed in most cells. Highly expressed in breast cancer cells.
  • Sequence similarities
    Contains 1 JmjC domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ13798 antibody
    • JmjC domain-containing protein 5 antibody
    • JMJD5 antibody
    • JMJD5 protein antibody
    • jumonji domain containing 5 antibody
    • Jumonji domain-containing protein 5 antibody
    • KDM8_HUMAN antibody
    • Lysine (K) specific demethylase 8 antibody
    • Lysine-specific demethylase 8 antibody
    see all

Images

  • Lane 1 : Anti-JMJD5 antibody (ab106391) at 1 µg/ml
    Lane 2 : Anti-JMJD5 antibody (ab106391) at 2 µg/ml

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 47 kDa
  • ab106391, at 2.5 µg/ml, staining JMJD5 in paraffin embedded Rat liver tissue by Immunohistochemistry.
  • Immunofluorescence of JMJD5 in Rat Liver cells using ab106391 at 20 ug/ml.

References

This product has been referenced in:
  • Wu BH  et al. Epigenetic silencing of JMJD5 promotes the proliferation of hepatocellular carcinoma cells by down-regulating the transcription of CDKN1A 686. Oncotarget 7:6847-63 (2016). Read more (PubMed: 26760772) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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