JMJD6 antibody (ab10526)

Product Name 

JMJD6 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to JMJD6

Immunogen 

Synthetic peptide conjugated to KLH, corresponding to amino acids 363-381 of Human PSR.

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IP: Use at an assay dependent dilution.
WB: 1/1000 using whole extracts of human HeLa or A549 cells. Detects a band of approximately 70 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Stem Cells >> Embryonic Stem Cells >> Intracellular
Immunology >> Innate Immunity >> Cytokines >> Other
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

Relevance 

JMJD6 (PSR / PTDSR) is required during embryogenesis and differentiation of multiple organs during embryogenesis. Probably acts as a key regulator of hematopoietic differentiation. May not be required for apoptotic cell clearance by macrophages but seems to be necessary for the regulation of macrophage cytokine responses.

JMJD6 (PSR / PTDSR) is a jumonji domain containing protein that may have a role in histone demethylation.

Most cells that undergo apoptosis are rapidly recognized, engulfed, and digested by professional phagocytes or by neighboring cells and eliminated from the organism. In this way, tissue exposure to potentially cytotoxic, immunogenic, or inflammatory cellular contents is prevented. As a result neither inflammation nor inappropriate immune responses are induced in this process. Removal of apoptotic cells comprise the following steps: surface alteration and ligand expression on the apoptotic cell, ligand recognition by tethering receptors on the phagocyte, initiation of appropriate signaling pathways in the phagocyte, engulfment and finally, digestion. Various molecules including proteins, carbohydrates, and phospholipids have been suggested to act as cell surface exposed markers for recognition by phagocytes. Virtually all apoptotic cells permanently express phosphatidylserine (PS) on the outer leaflet of their plasma membrane. Such loss of phospholipids asymmetry and exposure of PS does not occur in resting cells and appears only transiently following activation of certain cells. Phosphatidylserine Receptor (PSR) has been reported and shown to recognize phosphatidylserine in a stereospecific manner. PSR is an evolutionarily conserved glycoprotein that is widely expressed in cells and tissues. It is very susceptible to cleavage by protease or elastase. PSR engagement plays an important role in macropinocytosis and uptake of tethered apoptotic cells. Cooperation with other receptors and their ligands may also be needed for ingestion of apoptotic cells. PSR is also involved in suppression of inflammatory and immune responses.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 1.08 - 1.40 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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