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Western blot - JMJD6 antibody (ab64575)
Western blot - JMJD6 antibody (ab64575)
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Product Name 

JMJD6 peptide

Product type 

Proteins and Peptides

Peptide 

Synthetic peptide derived from within residues 350 to the C-terminus of Human JMJD6.This peptide was used as an immunogen for ab64575 - JMJD6 antibody.

Tested applications 
(see key)

BL

Application notes 
(see key)

Recommended dilutions
BL: Use at a concentration of 1 µg/ml.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Stem Cells >> Embryonic Stem Cells >> Intracellular
Immunology >> Innate Immunity >> Cytokines >> Other
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

Relevance 

JMJD6 (PSR / PTDSR) is required during embryogenesis and differentiation of multiple organs during embryogenesis. Probably acts as a key regulator of hematopoietic differentiation. May not be required for apoptotic cell clearance by macrophages but seems to be necessary for the regulation of macrophage cytokine responses.

JMJD6 (PSR / PTDSR) is a jumonji domain containing protein that may have a role in histone demethylation.

 

JMJD6 peptide (ab64574)

Purity 

70 - 90% by HPLC

Storage buffer 

Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this JMJD6 peptide is on this datasheet. But please do contact us if you would like any reassurance!

See below for JMJD6 peptide images, references, products related to ab64574 and other tools.
 

JMJD6 peptide images:

Product image  Western blot - JMJD6 antibody (ab64575)

Western blot - JMJD6 antibody (ab64575)

All lanes : JMJD6 antibody (ab64575) at 1/250 dilution

Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Liver (Rat) Tissue Lysate with JMJD6 peptide (ab64574) at 1 µg/ml
Lane 5 : Liver (Mouse) Tissue Lysate - normal tissue with JMJD6 peptide (ab64574) at 1 µg/ml
Lane 6 : Heart (Mouse) Tissue Lysate with JMJD6 peptide (ab64574) at 1 µg/ml

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 46 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)


The 70 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to JMJD6.
 
 

Search PubMed (MEDLINE) for references to JMJD6
 

JMJD6 peptide - more information

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Protocols for JMJD6 peptide

Price and availability of products related to JMJD6 peptide
 

JMJD6 peptide - related products:
 

Corresponding Antibody for JMJD6 peptide

ab64575

   

Rabbit polyclonal to JMJD6
 

Other JMJD6 Antibodies

See all JMJD6 antibodies (5)...
 

JMJD6 peptide - other tools:

Contact Abcam with a Technical Enquiry about JMJD6 peptide
 

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