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All lanes : Anti-JMY antibody (ab69945) at 1/500 dilution
Lane 1 : COLO cell exctract
Lane 2 : COLO cell exctract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 112 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
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